Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
France (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Human CD119
      Purified Mouse Anti-Human CD119
      Flow cytometric analysis of CD119 expresson on human PBMC (Left panel) and red blood cells (Right panel). The cells were isolated by Lymphoprep (Nycomed) density centrifugation before staining with Purified Mouse Anti-Human CD119 (1 µg, Cat. No. 558935; filled histograms) or left unstained (open histograms), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (0.25 µg, Cat. No. 553999) and PE Streptavidin (0.015 µg, Cat No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of intact cells.
      Purified Mouse Anti-Human CD119
      Flow cytometric analysis of CD119 expresson on human PBMC (Left panel) and red blood cells (Right panel). The cells were isolated by Lymphoprep (Nycomed) density centrifugation before staining with Purified Mouse Anti-Human CD119 (1 µg, Cat. No. 558935; filled histograms) or left unstained (open histograms), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (0.25 µg, Cat. No. 553999) and PE Streptavidin (0.015 µg, Cat No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of intact cells.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      IFN-γ Receptor α; IFN-γRα; IFNGR1; IFNGR; IFN-gamma receptor 1/IFN-gamma-R1
      Human (QC Testing)
      Mouse IgG2b, κ
      Human IFN-γRα
      Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
      0.5 mg/ml
      AB_397164
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      Immunofluorescent staining and flow cytometric analysis: Purified Mouse Anti-Human CD119 (Cat. No. 558935) can be used for the immunofluorescent staining (≤ 1 µg/ 10[6] cells) and flow cytometric analysis of the levels of membrane IFN-γRα expressed by human cell lines or human lymphoid cells. It is recommended that the purified format of this antibody be used in conjunction with Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat No. 553999) and PE Streptavidin (Cat No. 554061) in a three-layer staining procedure to amplify immunofluorescent signals.  An appropriate purified immunoglobulin isotype control is Purified Mouse IgG2b κ Isotype Control (Cat No. 555740).

      Since the GIR-94 is a non-neutralizing antibody, it can be used for immunofluorescent staining and flow cytometric analysis in systems where the IFN-γ ligand is present. Based on our testing results, the presence of recombinant human IFN-γ protein at levels below 1 µg/ml is insufficient to inhibit the binding of GIR-94 (at 0.06 µg mAb/1 million cells).

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      558935 Rev. 5
      Antibody Details
      Down Arrow Up Arrow
      GIR-94

      The GIR-94 antibody specifically recognizes the extracellular region of the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα; aka, CD119).  The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γR α and β chains required for the transduction of biologic responses.  The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium.  The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα  purified from human placenta.  The GIR-94 is a non-neutralizing antibody.

      558935 Rev. 5
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558935 Rev.5
      Citations & References
      Down Arrow Up Arrow

      Development References (5)

      1. Bach EA, Aguet M, Schreiber RD. The IFN gamma receptor: a paradigm for cytokine receptor signaling. Annu Rev Immunol. 1997; 15:563-591. (Biology). View Reference
      2. Greenlund AC, Schreiber RD, Goeddel DV, Pennica D. Interferon-gamma induces receptor dimerization in solution and on cells. J Biol Chem. 1997; 268(24):18103-18110. (Biology). View Reference
      3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:818-821.
      4. Sheehan KC, Calderon J, Schreiber RD. Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor. J Immunol. 1988; 140(12):4231-4237. (Immunogen). View Reference
      5. Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
      View All (5) View Less
      558935 Rev. 5

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback