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      APC-R700 Rat Anti-Mouse CD200
      APC-R700 Rat Anti-Mouse CD200
      Multicolor flow cytometric analysis of CD200 expression on mouse thymocytes. Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553047/553046/561835) and PE Rat Anti-Mouse CD8a (Cat. No. 553033/553032/561095) antibodies, and either BD Horizon™ APC-R700 Rat IgG2a, κ Isotype Control (Cat. No. 564982; dashed line histograms) or BD Horizon™ APC-R700 Rat Anti-Mouse CD200 antibody (Cat. No. 565546; solid line histograms). The fluorescence histograms showing CD200 expression (or Ig Isotype control staining) were derived from CD4-CD8a+ (Top Left Plot ), CD4+CD8a+ (Top Right Plot), CD4-CD8a- (Bottom Left Plot), or CD4+CD8a- (Bottom Right Plot) gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      APC-R700 Rat Anti-Mouse CD200
      Multicolor flow cytometric analysis of CD200 expression on mouse thymocytes. Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553047/553046/561835) and PE Rat Anti-Mouse CD8a (Cat. No. 553033/553032/561095) antibodies, and either BD Horizon™ APC-R700 Rat IgG2a, κ Isotype Control (Cat. No. 564982; dashed line histograms) or BD Horizon™ APC-R700 Rat Anti-Mouse CD200 antibody (Cat. No. 565546; solid line histograms). The fluorescence histograms showing CD200 expression (or Ig Isotype control staining) were derived from CD4-CD8a+ (Top Left Plot ), CD4+CD8a+ (Top Right Plot), CD4-CD8a- (Bottom Left Plot), or CD4+CD8a- (Bottom Right Plot) gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Product Details
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      BD Horizon™
      Cd200; OX2; OX-2; OX2G; MRC OX-2 antigen; Mox2
      Mouse (QC Testing)
      Rat IgG2a, κ
      Soluble fusion protein of the extracellular region of mouse OX-2 antigen w/domains 3 & 4 of rat CD4
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      17470
      AB_2739288
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      565546 Rev. 1
      Antibody Details
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      OX-90

      The OX-90 monoclonal antibody specifically recognizes CD200 (OX-2 antigen), a type-1 transmembrane glycoprotein containing two extracellular Ig-like domains. CD200 is expressed on thymocytes, neurons, B lymphocytes, splenic follicular dendritic cells and endothelium, and subsets of T lymphocytes and dendritic cells; but not on NK cells, granulocytes, monocytes, or macrophages. CD200 Receptor (CD200R; OX2R) expression is restricted to myeloid cells, and it appears that its engagement with CD200 results in inhibition and/or down-regulation of myeloid cell activity.

      This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor® 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor® 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor® (eg, 730/45-nm filter).

      565546 Rev. 1
      Format Details
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      APC-R700
      The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC-R700
      Red 627-640 nm
      651 nm
      706 nm
      565546 Rev.1
      Citations & References
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      View product citations for antibody "565546" on CiteAb

      Development References (4)

      1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
      2. Gorczynski L, Chen Z, Hu J, et al. Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells. J Immunol. 1999; 162(2):774-781. (Biology). View Reference
      3. Hoek RM, Ruuls SR, Murphy CA, et al. Down-regulation of the macrophage lineage through interaction with OX2 (CD200).. Science. 2000; 290(5497):1768-71. (Immunogen: ELISA, Flow cytometry, Immunohistochemistry). View Reference
      4. Preston S, Wright GJ, Starr K, Barclay AN, Brown MH. The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages. Eur J Immunol. 1997; 27(8):1911-1918. (Biology). View Reference
      View All (4) View Less
      565546 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.