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Purified Mouse anti-SLP-76 (pY128)
Purified Mouse anti-SLP-76 (pY128)
Western blot analysis of SLP-76 (pY128) in human T leukemia.  Lysates from control (left panel) and anti-CD3-activated (Cat. No. 555329, right panel) Jurkat cells were probed with purified mouse anti-SLP-76 (pY128) monoclonal antibody at concentrations of 0.03125 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6).  SLP-76 (pY128) is identified as a band of 76 kDa in the treated cells.
Western blot analysis of SLP-76 (pY128) in human T leukemia.  Lysates from control (left panel) and anti-CD3-activated (Cat. No. 555329, right panel) Jurkat cells were probed with purified mouse anti-SLP-76 (pY128) monoclonal antibody at concentrations of 0.03125 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6).  SLP-76 (pY128) is identified as a band of 76 kDa in the treated cells.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse BALB/c IgG1, κ
Phosphorylated Human SLP-76
Western blot (Routinely Tested)
76 kDa
0.5 mg/ml
AB_647331
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558367 Rev. 3
Antibody Details
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J141-668.36.58

SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR)-mediated intracellular signaling pathway.  It may be involved in the signaling pathways of other peripheral blood leukocytes; thymic/splenic cells; and in human T, B, and monocytic cell lines.  SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region.  SLP-76 has been shown to associate with Gads, Grb2, PLCγ1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes.  An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors.  The phosphorylated tyrosine 128 (Y128) brings the Rho family guanine nucleotide exchange factor Vav1 and the Nck adapter protein, which binds to p21-activated kinase (PAK1) and Wiskott-Aldrich syndrome protein (WASP), into the activation complex.  Vav1, PAK1, and WASP may mediate TCR-stimulated actin cytoskeletal rearrangement.  

The J141-688.36.58 monoclonal antibody recognizes the phosphorylated Y128 of activated SLP-76.

558367 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558367 Rev.3
Citations & References
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View product citations for antibody "558367" on CiteAb

Development References (3)

  1. Fang N, Motto DG, Ross SE, Koretzky GA. Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity. J Immunol. 1996; 157:3769-3773. (Biology). View Reference
  2. Janssen E, Zhang W. Adaptor proteins in lymphocyte activation. Curr Opin Immunol. 2003; 15:269-276. (Biology). View Reference
  3. Wu JN, Koretzky GA. The SLP-76 family of adapter proteins. Semin Immunol. 2004; 16:379-393. (Biology). View Reference
558367 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.