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HRP Goat Anti-Mouse Ig
Product Details
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BD Pharmingen™
Mouse (QC Testing)
ELISA (Routinely Tested), Western blot (Tested During Development)
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Preparation And Storage

Store undiluted at -20°C. Avoid multiple freeze-thaws of product. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.

The conjugate should be protected from prolonged exposure to light. The antibody solution was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross reactivity with rat, human, bovine, and horse serum proteins (multiple adsorption).For extended storage, aliquots of the antibody may be frozen after adding an equal volume of glycerol (which will decrease the antibody concentration).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. ProClin is a trademark of Rohm and Haas Company.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to for technical protocols.
554002 Rev. 21
Antibody Details
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HRP Goat Anti-Mouse Ig is a polyclonal secondary antibody used for the detection of mouse Ig.  This antibody is tested by ELISA.  Investigators are highly encouraged to titrate the reagent for specific applications (a 1:1000 dilution is the suggested starting dilution). For immunohistochemical staining, biotin goat anti-mouse Ig (Cat. No. 550337) is recommended.

Warning:  HRP Goat Anti-Mouse Ig (Cat. No. 554002) contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

554002 Rev. 21
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554002 Rev.21
Citations & References
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554002 Rev. 21

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.