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Purified Mouse Anti-NMDAR2A
Product Details
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BD Transduction Laboratories™
N-Methyl D-Aspartate
Mouse (QC Testing), Rat (Tested in Development)
Mouse IgG1
Mouse NMDAR2A aa. 1093-1214
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
180 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612286 Rev. 1
Antibody Details
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The majority of synapses in the central nervous system utilize glutamate as a neurotransmitter to produce rapid neuronal excitation. Glutamate has a diverse array of receptors that can be categorized into two groups, ionotropic and metabotropic. The ionotropic receptors are subdivided into two distinct types: 1) receptors for N-methyl D-aspartate (NMDAR) and 2) non-NMDA receptors for AMPA and kainate. Four types of NMDAR2 have been identified: NMDAR2A (NR2A), NR2B, NR2C, and NR2D. NR2A and NR2B contain a C-terminal extension (> 600 amino acids) that has small, scattered regions of conserved sequence. In embryonic stages, NR2B is expressed throughout the brain, while NR2D is found in diencephalon and brainstem. After birth, NR2A is found throughout the brain, and NR2C is expressed in cerebellum. At this time, NR2B expression becomes restricted to the forebrain, and NR2D expression is strongly reduced. Deletion of the most widespread postnatal NR subunit, NR2A, does not have adverse affects on brain development, but reduces long-term potentiation (LTP) in the hippocampus and impairs spatial and contextual learning. Thus, NR2A may be critical for postnatal learning.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612286 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612286 Rev.1
Citations & References
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View product citations for antibody "612286" on CiteAb

Development References (2)

  1. Ishii T, Moriyoshi K, Sugihara H, et al. Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J Biol Chem. 1993; 268(4):2836-2843. (Biology). View Reference
  2. Morikawa E, Mori H, Kiyama Y, Mishina M, Asano T, Kirino T. Attenuation of focal ischemic brain injury in mice deficient in the epsilon1 (NR2A) subunit of NMDA receptor. J Neurosci. 1998; 18(23):9727-9732. (Biology). View Reference
612286 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.