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      Purified Mouse Anti-Sec8
      Purified Mouse Anti-Sec8
      Western blot analysis of Sec8 on a MDCK cell lysate (Canine kidney; ATCC CCL-34). Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of the mouse anti-Sec8 antibody.
      Purified Mouse Anti-Sec8
      Immunofluorescence of AN3 CA cells (Human endometrial adenocarcinoma; ATCC HTB-111).
      Purified Mouse Anti-Sec8 Purified Mouse Anti-Sec8
      Western blot analysis of Sec8 on a MDCK cell lysate (Canine kidney; ATCC CCL-34). Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of the mouse anti-Sec8 antibody.
      Immunofluorescence of AN3 CA cells (Human endometrial adenocarcinoma; ATCC HTB-111).
      Product Details
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      BD Transduction Laboratories™
      Dog (QC Testing), Human, Mouse, Rat, Chicken (Tested in Development)
      Mouse IgG2b
      Rat Sec8 aa. 31-201
      Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
      110 kDa
      250 µg/ml
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      610658 Rev. 1
      Antibody Details
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      14/Sec8

      Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process, which is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, leads to membrane fusion and neurotransmitter secretion. Sec8 is a hydrophilic protein of 975 amino acids that is highly expressed in brain and kidney. It is homologus with the yeast  secretory protein, Sec8p. In yeast, Sec8 is essential for the Golgi-to-plasma membrane traffic of proteins during constitutive secretion. In rat brain, Sec8 colocalizes with Rab3 and Syntaxin-1a, two important proteins that regulate the fusion of the synaptic vesicle to the plasma membrane. The primary structure of Sec8, a coiled-coil domain (residues 34-99), is commonly found in other proteins important in secretory pathways.

      This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      610658 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610658 Rev.1
      Citations & References
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      Development References (4)

      1. Charron AJ, Nakamura S, Bacallao R, Wandinger-Ness A. Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells. J Cell Biol. 2000; 149(1):111-124. (Biology: Immunofluorescence, Western blot). View Reference
      2. Duncan RR, Don-Wauchope AC, Tapechum S, Shipston MJ, Chow RH, Estibeiro P. High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells. Biochem J. 1999; 342(Pt 3):497-501. (Biology: Western blot). View Reference
      3. Hazuka CD, Hsu SC, Scheller RH. Characterization of a cDNA encoding a subunit of the rat brain rsec6/8 complex. Gene. 1997; 187(1):67-73. (Biology). View Reference
      4. Ting AE, Hazuka CD, Hsu SC. rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion. Proc Natl Acad Sci U S A. 1995; 92(21):9613-9617. (Biology). View Reference
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      610658 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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