Western Blot analysis of NeuroD1 in human Retinoblastoma cell lysate. Lysate from Y79 cells (ATCC, HTB-18™) was probed with Purified Mouse Anti-NeuroD1 monoclonal antibody at titrations of 0.125 (lane 1), 0.06 (lane 2), and 0.03 μg/ml (lane 3). NeuroD1 is identified as a band of approximately 45 kDa.
Immunoflourescent staining of NeuroD1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1% Triton™ X-100 Buffer, and stained with Purified Mouse Anti-NeuroD1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
Western Blot analysis of NeuroD1 in human Retinoblastoma cell lysate. Lysate from Y79 cells (ATCC, HTB-18™) was probed with Purified Mouse Anti-NeuroD1 monoclonal antibody at titrations of 0.125 (lane 1), 0.06 (lane 2), and 0.03 μg/ml (lane 3). NeuroD1 is identified as a band of approximately 45 kDa.
Immunoflourescent staining of NeuroD1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1% Triton™ X-100 Buffer, and stained with Purified Mouse Anti-NeuroD1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
Product Details
BD Pharmingen™
Class A basic helix-loop-helix protein 3, bHLHa3, BETA2, NeuroD
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1, κ
Human NeuroD1 Recombinant Protein
Western blot (Routinely Tested), Bioimaging, Immunofluorescence, Intracellular staining (flow cytometry) (Tested During Development)
~45 kDa
0.5 mg/ml
AB_2737942
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Triton is a trademark of the Dow Chemical Company.
- All other brands are trademarks of their respective owners.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
HRP Goat Anti-Mouse Ig RUO
Size
1 mL
Cat No.
554002
563000 Rev. 1
Antibody Details
R8-294
The R8-294 monoclonal antibody specifically binds to Neurogenic differentiation factor 1 (NeuroD1). NeuroD1 is a basic helix-loop-helix (bHLH) transcription factor that is also known as BHLHA3, Beta-cell E-box transactivator 2, BETA2 and NeuroD. NeuroD1 interacts with other bHLH proteins and binds the insulin E-box sequence to regulate pancreatic islet cell development. It also plays a key role in central nervous system (CNS) and sensory nervous system neuron development. Specifically, it is required for the terminal differentiation of neurons during late stages of neurogenesis in the CNS (cerebral cortex, hippocampus, and cerebellum) and the sensory nervous system (eye, inner ear, and olfactory system). During antibody development, the purified R8-294 monoclonal antibody was found to detect NeuroD1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis.
563000 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
563000 Rev.1
Citations & References
View product citations for antibody "563000" on CiteAb
Development References (9)
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Cherry TJ, Wang S, Bormuth I, Schwab M, Olson J, Cepko CL. NeuroD factors regulate cell fate and neurite stratification in the developing retina. J Neurosci. 2011; 31(20):7365-7379. (Biology). View Reference
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Cho JH, Tsai MJ. The role of BETA2/NeuroD1 in the development of the nervous system. Mol Neurobiol. 2004; 30(1):35-47. (Biology). View Reference
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Gao Z, Ure K, Ables JL, et al. Neurod1 is essential for the survival and maturation of adult-born neurons. Nat Neurosci. 2009; 12(9):1090-1092. (Biology). View Reference
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Habener JF, Kemp DM, Thomas MK. Minireview: transcriptional regulation in pancreatic development. Endocrinology. 2005; 146(3):1025-1034. (Biology). View Reference
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Kuwabara T, Hsieh J, Muotri A, et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci. 2009; 12(9):1097-10105. (Biology). View Reference
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Naya FJ, Huang HP, Qiu Y, et al. Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/neuroD-deficient mice. Genes Dev. 1997; 11(18):2323-2334. (Biology). View Reference
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Naya FJ, Stellrecht CM, Tsai MJ. Tissue-specific regulation of the insulin gene by a novel basic helix-loop-helix transcription factor. Genes Dev. 1995; 9(8):1009-1019. (Biology). View Reference
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Pang ZP, Yang N, Vierbuchen T, et al. Induction of human neuronal cells by defined transcription factors. Nature. 2011; 476(7359):220-223. (Biology). View Reference
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Tamimi R, Steingrimsson E, Copeland NG, et al. The NEUROD gene maps to human chromosome 2q32 and mouse chromosome 2. Genomics. 1996; 15(34):418-421. (Biology). View Reference
563000 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.