BD Pharmingen™ Purified Mouse Anti-Human CD106
Clone 51-10C9 (RUO)
Flow cytometric analysis of CD106 expression on TNF-α-stimulated HUVEC cell line. HUVEC cells were stimulated with Recombinant Human TNF Protein (Cat. No. 554618; 20 ng/ml), then stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD106 (Cat. No. 555645; solid line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of CD106 expression on TNF-α-stimulated HUVEC cell line. HUVEC cells were stimulated with Recombinant Human TNF Protein (Cat. No. 554618; 20 ng/ml), then stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD106 (Cat. No. 555645; solid line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ system.
Product Details
BD Pharmingen™
VCAM-1; Vascular cell adhesion protein 1; INCAM-100; L1CAM
Human (QC Testing)
Mouse IgG1, κ
Human VCAM-1 Recombinant Protein
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, ELISA, Immunoprecipitation (Reported)
0.5 mg/ml
V E112
AB_396002
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Purified Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555746
Recombinant Human TNF RUO
Size
10 µg
Cat No.
554618
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
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555645 Rev. 2
Antibody Details
51-10C9
The 51-10C9 monoclonal antibody specifically binds to CD106. CD106 is a 100-110 kDa type I transmembrane sialoglycoprotrein that is also known as Vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110. CD106 is expressed at high levels on the surface of cytokine-stimulated endothelium, and at minimal levels on unstimulated endothelium. VCAM-1 serves as a ligand for the leukocyte integrins α4β1 (CD49d/CD29 complex; VLA-4) and α4β7 (LPAM-1). The 51-10C9 monoclonal antibody inhibits the in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.
555645 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555645 Rev.2
Citations & References
View product citations for antibody "555645" on CiteAb
Development References (4)
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Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA Jr. Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci U S A. 1987; 84(24):9238-9242. (Biology). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Taichman DB, Cybulsky MI, Djaffar I, et al. Tumor cell surface alpha 4 beta 1 integrin mediates adhesion to vascular endothelium: demonstration of an interaction with the N-terminal domains of INCAM-110/VCAM-1. Cell Regul. 1991; 2(5):347-355. (Biology). View Reference
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van Vugt MJ, van den Herik-Oudijk IE, van de Winkle JG. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996; 88(6):2358-2361. (Immunogen). View Reference
555645 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.