PE Rat Anti-Mouse CD8b

BD Pharmingen™ PE Rat Anti-Mouse CD8b

Name: PE Rat Anti-Mouse CD8b
Brand: BD Pharmingen™
SKU: 568906

Clone YTS156.7.7.rMAb (also known as YTS156.7.7; YTS156)

(RUO)

Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066) and with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse CD8b antibody (Cat. No. 568906/568907; Right Plot) at 0.25 μg/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b (or Ig Isotype control staining) versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Cd8b1; Cd8b; Ly-3; Lyt-3; lymphocyte antigen 3

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: Mouse Thymocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Rat IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 553925

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.5 mg Cat No. 553142

APC Hamster Anti-Mouse CD3e RUO

Size 0.1 mg Cat No. 553066

Lysing Buffer RUO

Size 100 mL Cat No. 555899

View Details

568906 Rev. 1

Antibody Details

YTS156.7.7.rMAb

The YTS156.7.7.rMAb is a recombinant monoclonal antibody derived from YTS156.7.7 hybridoma cells that specifically recognizes the β chain of the CD8 differentiation antigen (CD8b), which is also known as Lymphocyte antigen 3 (Ly-3) or Lyt-3. CD8b is a single-pass type I transmembrane glycoprotein that is encoded by Cd8b1 (CD8 antigen, beta chain 1) which belongs to the Ig superfamily. CD8b is comprised of an N-terminal IgV domain in its extracellular region followed by a transmembrane region and a cytoplasmic tail. CD8b can bind to the CD8 α chain (CD8a) to form the disulfide-linked CD8 αβ (CD8ab) heterodimer also known as Ly2,3 or Lyt-2,3. This heterodimer is expressed on most thymocytes and a subpopulation of MHC class I-restricted mature T cells. CD8 αβ functions as an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and the protein tyrosine kinase, lck (p56lck).

568906 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

568906 Rev.1

Citations & References

View product citations for antibody "568906" on CiteAb

Development References (5)

Cobbold S, Martin G, Waldmann H. Monoclonal antibodies for the prevention of graft-versus-host disease and marrow graft rejection. The depletion of T cell subsets in vitro and in vivo.. Transplantation. 1986; 42(3):239-47. (Immunogen: Flow cytometry). View Reference
Liunggren G, Liunggren HG, Dalianis T. T cell subsets involved in immunity against polyoma virus-induced tumors.. Virology. 1994; 198(2):714-6. (Clone-specific: In vivo exacerbation). View Reference
Monney L1, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease.. Nature. 2002; 415(6871):536-541. (Clone-specific: Flow cytometry). View Reference
Shore DA, Issafras H, Landais E, Teyton L, Wilson IA. The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.. J Mol Biol. 2008; 384(5):1190-202. (Clone-specific: Blocking). View Reference
Wei Y, Chen K, Sharp GC, Yagita H, Braley-Mullen H. Expression and regulation of Fas and Fas ligand on thyrocytes and infiltrating cells during induction and resolution of granulomatous experimental autoimmune thyroiditis.. J Immunol. 2001; 167(11):6678-86. (Clone-specific: In vivo exacerbation). View Reference

View All (5)

568906 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.