PE Mouse Anti-Mouse TCR Vγ4

BD Pharmingen™ PE Mouse Anti-Mouse TCR Vγ4

Name: PE Mouse Anti-Mouse TCR Vγ4
Brand: PE Mouse Anti-Mouse TCR Vγ4
SKU: 569158

Clone 49.2 (also known as 49.2-9) (RUO)

Multicolor flow cytometric analysis of TCR Vγ4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with BD Optibuild™ R718 Hamster Anti-Mouse γδ T-Cell Receptor (Cat. No. 751919), FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061) and with either PE Mouse IgG1, k Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Mouse TCR Vγ4 (Cat. No. 569158/569159; Right Plot) at 1 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vγ4 (or Ig Isotype control staining) versus TCRγδ was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: T Cell Receptor Vγ4; TCR Vg4; TCR Vgamma4; TCR Vγ2 (Garman Nomenclature); Tcrg-V4; Trgv4

Reactivity: Mouse (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Mouse TCR γδ Hybridoma Cells

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 21638

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

R718 Hamster Anti-Mouse γδ T-Cell Receptor RUO

Size 50 µg Cat No. 751919

FITC Hamster Anti-Mouse CD3e RUO

Size 0.5 mg Cat No. 553062

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

View Details

569158 Rev. 3

Antibody Details

49.2

The 49.2 monoclonal antibody specifically recognizes the variable gamma 4 region of the γ subunit of the mouse γδ T cell receptor for antigen, TCR Vγ4 (using the Heilig and Tonegawa nomenclature for mouse TCR γ and δ chains). TCR Vγ4 is encoded by the Trgv4 (T cell receptor gamma, variable 4) gene element. TCR Vγ4 is expressed by a subset of TCR γδ+ thymocytes in the late fetal and adult thymus and by γδ T cells in peripheral lymphoid and non-lymphoid tissues. The frequency of splenic TCR Vγ4+ γδ T cells may differ among inbred mouse strains. The 49.2 antibody is useful for TCR Vγ4+ thymocyte and γδ T cell separations and analyzing TCR Vγ repertoires expressed by thymocytes, peripheral T cells, and T cell hybridomas in developmental and other experimental model systems. The UC3-10A6 antibody also recognizes the TCR Vγ4 segment which is referred to as TCR Vγ2 using the Garman, Doherty, and Raulet nomenclature for mouse TCR γ and δ chains.

569158 Rev. 3

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

569158 Rev.3

Citations & References

View product citations for antibody "569158" on CiteAb

Development References (6)

Garman RD, Doherty PJ, Raulet DH. Diversity, rearrangement, and expression of murine T cell gamma genes.. Cell. 1986; 45(5):733-42. (Biology). View Reference
Heilig JS, Tonegawa S. Diversity of murine gamma genes and expression in fetal and adult T lymphocytes.. Nature. 322(6082):836-40. (Biology). View Reference
Kashani E, Föhse L, Raha S, et al. A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset.. Nat Commun. 2015; 6:6477. (Clone-specific: Flow cytometry). View Reference
Pereira P, Boucontet L. Rates of recombination and chain pair biases greatly influence the primary gammadelta TCR repertoire in the thymus of adult mice.. J Immunol. 2004; 173(5):3261-70. (Clone-specific: Flow cytometry). View Reference
Pereira P, Hermitte V, Lembezat MP, Boucontet L, Azuara V, Grigoriadou K. Developmentally regulated and lineage-specific rearrangement of T cell receptor Valpha/delta gene segments.. Eur J Immunol. 2000; 30(7):1988-97. (Immunogen: Flow cytometry). View Reference
Sell S, Dietz M, Schneider A, Holtappels R, Mach M, Winkler TH. Control of murine cytomegalovirus infection by γδ T cells.. PLoS Pathog. 2015; 11(2):e1004481. (Clone-specific: Flow cytometry). View Reference

View All (6)

569158 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.