PE Mouse anti-Mouse IL-23 Receptor

BD Pharmingen™ PE Mouse anti-Mouse IL-23 Receptor

Name: PE Mouse anti-Mouse IL-23 Receptor
Brand: BD Pharmingen™
SKU: 562468

Clone 3C9

(RUO)

Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, dashed line histogram) or PE Mouse anti-Mouse IL-23 Receptor (Cat. No. 562468; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Il23r; IL-23R; Interleukin 23 receptor

Reactivity: Mouse (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Mouse IL-23 Receptor Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 209590

RRID: AB_11154593

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

562468 Rev. 2

Antibody Details

3C9

The 3C9 monoclonal antibody specifically binds to the mouse Interleukin-23 Receptor (IL-23R) subunit that is encoded by the il23r gene. The IL-23R subunit is a type I transmembrane glycoprotein and member of the hemopoietin receptor superfamily. The mouse IL-23 Receptor complex is comprised of IL-23R and IL-12 receptor beta 1 (IL-12Rβ1) subunits. The IL-23R complex can bind IL-23, a cytokine that plays roles in innate and adaptive immunity as well as in autoimmune diseases, eg, by the generation and maintenance of Th17 cells. Mouse IL-23R is expressed by activated/memory CD4+ T cells, Th1, Th2 and Th17 cells, γδ T cells, dendritic cells and macrophages as determined by IL-23R mRNA expression and IL-23R-GFP reporter mouse studies. The IL-23-bound IL-23R complex transduces an intracellular signal pathway mediated by a Jak-STAT signaling cascade.

562468 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

562468 Rev.2

Citations & References

View product citations for antibody "562468" on CiteAb

Development References (6)

Awasthi A, Riol-Blanco L, Jager A, et al. Cutting edge: IL-23 receptor gfp reporter mice reveal distinct populations of IL-17-producing cells. J Immunol. 2009; 182(10):5904-5908. (Biology). View Reference
Jones LL, Alli R, Li B, Geiger TL. Differential T Cell Cytokine Receptivity and Not Signal Quality Distinguishes IL-6 and IL-10 Signaling during Th17 Differentiation.. J Immunol. 2016; 196(7):2973-85. (Biology). View Reference
Lankford CS, Frucht DM. A unique role for IL-23 in promoting cellular immunity. J Leukoc Biol. 2003; 73(1):49-56. (Biology). View Reference
Monk JM, Hou TY, Turk HF, McMurray DN, Chapkin RS. n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells.. J Nutr. 2013; 143(9):1501-8. (Biology). View Reference
Parham C, Chirica M, Timans J, et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002; 168(11):5699-5708. (Biology). View Reference
Petermann F, Rothhammer V, Claussen MC, et al. gammadelta T cells enhance autoimmunity by restraining regulatory T cell responses via an interleukin-23-dependent mechanism. Immunity. 2010; 33(3):351-363. (Biology). View Reference

View All (6)

562468 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.