Flow cytometric analysis of CD11a expression on human peripheral blood lymphocytes. Human whole blood was stained with either PE Mouse Anti-Human CD11a (Cat. No. 555380; solid line histogram) or PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of CD11a expression on human peripheral blood lymphocytes. Human whole blood was stained with either PE Mouse Anti-Human CD11a (Cat. No. 555380; solid line histogram) or PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Product Details
BD Pharmingen™
LFA-1α; LFA-1A; LFA1A; Integrin alpha-L; ITGAL
Human (QC Testing)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
20 µl
AB_395781
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
PE Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
555574
555380 Rev. 12
Antibody Details
G43-25B
The G43-25B monoclonal antibody specifically binds to CD11a. CD11a is the 180 kD integrin α chain which associates with CD18 (integrin β2) to form the heterodimer glycoprotein CD11a/CD18. This is known as the lymphocyte function associated antigen-1 (LFA-1), which is on all leukocytes; and is an adhesion molecule involved in lymphocyte and granulocyte functions. LFA-1 mediates adhesion of lymphoid cells to the vascular endothelium in association with its ligand, the intracellular adhesion molecule-1 (ICAM-1), CD54. Other ligands are ICAM-2 (CD102) and ICAM-3 (CD50).
555380 Rev. 12
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555380 Rev.12
Citations & References
View product citations for antibody "555380" on CiteAb
Development References (3)
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Dustin ML, Springer TA. Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. J Cell Biol. 1988; 107(1):321-331. (Biology). View Reference
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Dzionek A et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.. J Immunol. 2000; 165(11):6037-6046. (Clone-specific). View Reference
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Rothlein R, Dustin ML, Marlin SD, Springer TA. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J Immunol. 1986; 137(4):1270-1274. (Biology). View Reference
555380 Rev. 12
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.