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      PE-CF594 Mouse Anti-Human LAG-3 (CD223)
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      Improve data resolution with PE-CF594 alternative with reduced spillover and enhanced brightness! Try BD RealBlue™ 613 SKU# [758681] today! More Info on RB613 #
      PE-CF594 Mouse Anti-Human LAG-3 (CD223)
      Multicolor flow cytometric analysis of LAG-3 (CD223) expression on unstimulated and stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were cultured for 3 days with plate-bound Anti-Human CD3 (Cat. No. 555329; 10 µg/mL for coating) and soluble Anti-Human CD28 (Cat. No. 555725; 1 µg/mL) antibodies, and Human Recombinant IL-2 (Cat. No. 554603; 10 ng/mL). Unstimulated (Top Plots) or stimulated (Bottom Plots) PBMC from the same donor were stained with APC Mouse Anti-Human CD8 (Cat. No. 555369/561421/561952/561953), BD Horizon™ BV421 Mouse Anti-Human CD279 (PD-1) (Cat. No. 562516/565935), and BD Horizon™ PE-CF594 Mouse Anti-Human LAG-3 (CD223) (Cat. No. 565718/565719) antibodies. Two-color flow cytometric contour plots showing the correlated expression of LAG-3 (CD223) versus CD8 (Left Plots), or LAG-3 (CD223) versus CD279 (PD-1) (Right Plots) were derived from gated events with the forward and side light-scatter characteristics of viable unstimulated (Top Plots) or stimulated (Bottom Plots) lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      PE-CF594 Mouse Anti-Human LAG-3 (CD223)
      Multicolor flow cytometric analysis of LAG-3 (CD223) expression on unstimulated and stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were cultured for 3 days with plate-bound Anti-Human CD3 (Cat. No. 555329; 10 µg/mL for coating) and soluble Anti-Human CD28 (Cat. No. 555725; 1 µg/mL) antibodies, and Human Recombinant IL-2 (Cat. No. 554603; 10 ng/mL). Unstimulated (Top Plots) or stimulated (Bottom Plots) PBMC from the same donor were stained with APC Mouse Anti-Human CD8 (Cat. No. 555369/561421/561952/561953), BD Horizon™ BV421 Mouse Anti-Human CD279 (PD-1) (Cat. No. 562516/565935), and BD Horizon™ PE-CF594 Mouse Anti-Human LAG-3 (CD223) (Cat. No. 565718/565719) antibodies. Two-color flow cytometric contour plots showing the correlated expression of LAG-3 (CD223) versus CD8 (Left Plots), or LAG-3 (CD223) versus CD279 (PD-1) (Right Plots) were derived from gated events with the forward and side light-scatter characteristics of viable unstimulated (Top Plots) or stimulated (Bottom Plots) lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Product Details
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      BD Horizon™
      LAG3; CD223; FDC; Lymphocyte activation gene 3 protein; Protein FDC
      Human (QC Testing)
      Mouse IgG1, κ
      Human LAG-3 Recombinant Protein
      Flow cytometry (Routinely Tested)
      5 µl
      3902
      AB_2869706
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      6. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
      7. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
      8. CF™ is a trademark of Biotium, Inc.
      9. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      10. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      11. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      12. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      565719 Rev. 1
      Antibody Details
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      T47-530

      The T47-530 specifically recognizes the Lymphocyte Activation Gene 3 (LAG-3) protein which is also known as, Protein FDC, or CD223. LAG-3 is a ~70 kDa type I transmembrane glycoprotein that belongs to the Ig superfamily and exhibits homology to CD4. LAG-3 is expressed on NK cells, regulatory T cells, and activated conventional T cells with higher expression found on CD8+ T cells compared with CD4+ T cells. LAG-3 is an activation induced cell surface molecule that like CD4, binds MHC class II molecules, but with much higher affinity. This may enable LAG-3 to act as a negative competitor of CD4 for MHC class II ligand binding. LAG-3 may associate with the TCR-CD3 complex to downregulate TCR signal transduction and T cell clonal expansion. In contrast, LAG-3-induced signaling may promote dendritic cell activation.

      This antibody is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

      565719 Rev. 1
      Format Details
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      PE-CF594
      BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE-CF594
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      615 nm
      565719 Rev.1
      Citations & References
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      View product citations for antibody "565719" on CiteAb

      Development References (5)

      1. Casati C, Camisaschi C, Novellino L, et al. Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation. J Immunol. 2008; 180(6):3782-3788. (Biology). View Reference
      2. Hannier S, Tournier M, Bismuth G, Triebel F. CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling. J Immunol. 1998; 161(8):4058-4065. (Biology). View Reference
      3. Huang CT, Workman CJ, Flies D, et al. Role of LAG-3 in regulatory T cells. Immunity. 2004; 21(4):503-513. (Biology). View Reference
      4. Triebel F, Hacene K, Pichon MF. A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors. Cancer Lett. 2006; 235(1):147-153. (Biology). View Reference
      5. Triebel F, Jitsukawa S, Baixeras E, et al. LAG-3, a novel lymphocyte activation gene closely related to CD4. J Exp Med. 1990; 171(5):1393-1405. (Biology). View Reference
      View All (5) View Less
      565719 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.