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BV421 Rat Anti-Mouse IgG1
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BV421 Rat Anti-Mouse IgG1
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse IgG1 antibody (Cat. No. 742475; solid line histogram) as second step antibody. Left: Viable Lewis rat spleen cells were first stained with (solid line histogram) or without (dotted line histogram) Purified Mouse Anti-Rat CD45RA (clone OX-33) antibody. Right: Viable C57BL/6 mouse spleen cells were first stained with (solid line histogram) or without (dotted line histogram) Purified Mouse Anti-Mouse CD22 (clone Cy34.1) antibody.  Flow cytometry of rat tissue was performed using a BD LSRFortessa™ Flow Cytometer System. Flow cytometry of mouse tissue was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse IgG1 antibody (Cat. No. 742475; solid line histogram) as second step antibody. Left: Viable Lewis rat spleen cells were first stained with (solid line histogram) or without (dotted line histogram) Purified Mouse Anti-Rat CD45RA (clone OX-33) antibody. Right: Viable C57BL/6 mouse spleen cells were first stained with (solid line histogram) or without (dotted line histogram) Purified Mouse Anti-Mouse CD22 (clone Cy34.1) antibody.  Flow cytometry of rat tissue was performed using a BD LSRFortessa™ Flow Cytometer System. Flow cytometry of mouse tissue was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
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BD OptiBuild™
Igh-4; Ighg1; Immunoglobulin heavy constant gamma 1
Mouse (Tested in Development)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Mouse IgG1, κ Antibody
Flow cytometry (Qualified)
0.2 mg/ml
16017
AB_2740809
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
742475 Rev. 2
Antibody Details
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X56

The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not crossreact with other Ig isotypes.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

742475 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
742475 Rev.2
Citations & References
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View product citations for antibody "742475" on CiteAb

Development References (3)

  1. Hao Z, Duncan GS, Su YW, et al.. The E3 ubiquitin ligase Mule acts through the ATM-p53 axis to maintain B lymphocyte homeostasis. J Exp Med. 2012; 209(1):173-186. (Clone-specific: Flow cytometry). View Reference
  2. Kita-Furuyama M, Nagayama Y, Pichurin P, McLachlan SM, Rapoport B, Eguchi K. Dendritic cells infected with adenovirus expressing the thyrotrophin receptor induce Graves' hyperthyroidism in BALB/c mice. Clin Exp Immunol. 2003; 131(2):234-240. (Clone-specific: ELISA). View Reference
  3. Vuong BQ, Lee M, Kabir S, et al. Specific recruitment of protein kinase A to the immunoglobulin locus regulates class-switch recombination.. Nat Immunol. 2009; 10(4):420-6. (Clone-specific: Flow cytometry). View Reference
742475 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.