Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Ultraviolet 737 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
Companion Products
The MHD4-46 monoclonal antibody specifically binds to Delta-Like Protein 4 which is also known as Delta4, DLL4, and DL4. Delta-Like Protein 4 is a type 1 transmembrane glycoprotein which belongs to the Delta/Serrate/Jagged Family of Notch receptor ligands. The Notch family of transmembrane receptors and their ligands play key roles in various developmental and postnatal processes by controlling cell-fate decisions. Delta-Like Protein 4 is expressed by vascular endothelial cells within certain normal tissues and tumors. It signals through Notch1 and Notch 4 Receptors and plays a critical role in vascular development and homeostasis. Delta-Like Protein 4 expression is upregulated in response to vascular endothelial growth factor (VEGF) and regulates endothelial cell proliferation during vascular sprouting. Delta-Like Protein 4 is also expressed by activated human monocytes and dendritic cells that have been stimulated with proinflammatory activators such as lipopolysaccharide. Recent evidence suggests that Delta-Like Protein 4 may contribute to the T cell potential of CD34+ cells and to the growth of various tumors, and promote tumor cell infiltration.
The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.
Development References (10)
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Artavanis-Tsakonas S, Rand MD, Lake RJ. Notch signaling: cell fate control and signal integration in development.. Science. 1999; 284(5415):770-6. (Biology). View Reference
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Fung E, Tang SM, Canner JP, et al. Delta-like 4 induces notch signaling in macrophages: implications for inflammation. Circulation. 207; 115(23):2948-2956. (Biology). View Reference
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Gurney A, Hoey T. Anti-DLL4, a cancer therapeutic with multiple mechanisms of action. Vasc Cell. 2011; 3(18):21. (Biology). View Reference
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Herr F, Schreiner I, Baal N, Pfarrer C, Zygmunt M. Expression patterns of Notch receptors and their ligands Jagged and Delta in human placenta. Placenta. 2011; 32(8):554-563. (Biology). View Reference
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Lefort N, Benne C, Lelievre JD, et al. Short exposure to Notch ligand Delta-4 is sufficient to induce T-cell differentiation program and to increase the T cell potential of primary human CD34+ cells. Exp Hematol. 2006; 34(12):1720-1729. (Biology). View Reference
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Mailhos C, Modlich U, Lewis J, Harris A, Bicknell R, Ish-Horowicz D. Delta4, an endothelial specific notch ligand expressed at sites of physiological and tumor angiogenesis. Differentiation. 2001; 69(2-3):135-144. (Biology). View Reference
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Sekine C, Koyanagi A, Koyama N, Hozumi K, Chiba S, Yagita H. Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. Arthritis Res Ther. 2012; 14(2):R45-R58. (Immunogen). View Reference
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Shutter JR, Scully S, Fan W, et al. Dll4, a novel Notch ligand expressed in arterial endothelium. Genes Dev. 2000; 14:1313-1318. (Biology). View Reference
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Shutter JR, Scully S, Fan W, et al. Molecular cloning of delta-4, a new mouse and human Notch ligand. J Biochem (Tokyo). 2001; 129(1):27-34. (Biology). View Reference
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Wakui M, Nakano K, Matsushita S. Notch ligand mRNA levels of human APCs predict Th1/Th2-promoting activities. Biochem Biophys Res Commun. 2007; 358(2):596-601. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.