Multicolor flow cytometric analysis of KIR2DS4 (CD158i) expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD56 (Cat No. 555516/561903) and FITC Mouse Anti-Human CD3 (Cat No. 555332/561806/561807) antibodies, and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human KIR2DS4 (CD158i) antibody (Cat. No. 564375; Middle and Right Plots). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric contour plots show the correlated expression patterns of CD56 (Left and Middle Plots) or CD3 (Right Plot) versus KIR2DS4 (CD158i) [or Ig Isotype control staining] for gated events with the forward and side light-scatter characteristics of viable peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of KIR2DS4 (CD158i) expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD56 (Cat No. 555516/561903) and FITC Mouse Anti-Human CD3 (Cat No. 555332/561806/561807) antibodies, and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human KIR2DS4 (CD158i) antibody (Cat. No. 564375; Middle and Right Plots). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric contour plots show the correlated expression patterns of CD56 (Left and Middle Plots) or CD3 (Right Plot) versus KIR2DS4 (CD158i) [or Ig Isotype control staining] for gated events with the forward and side light-scatter characteristics of viable peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
KIR2DS4; KIR antigen 2DS4; KKA3; NKAT8; p58 NK receptor CL-39/CL-1
Human (QC Testing)
Mouse IgG2a, κ
Human KIR2DS4-Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2738776
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
557715
PE Mouse Anti-Human CD56 (NCAM-1) RUO
Size
100 Tests
Cat No.
555516
PE Mouse Anti-Human CD56 (NCAM-1) RUO
Size
25 Tests
Cat No.
561903
564375 Rev. 1
Antibody Details
179315
The 179315 monoclonal antibody specifically binds to Killer cell immunoglobulin-like receptor 2DS4 which is also known as CD158i, KKA3, and NKAT8. CD158i is encoded by KIR2DS4 (killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4). It is a type I transmembrane glycoprotein that can bind to HLA-C antigens and function as an activating immunoglobulin-like receptor that is expressed on NK cells and some T cells.
564375 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564375 Rev.1
Citations & References
View product citations for antibody "564375" on CiteAb
Development References (4)
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Beziat V, Liu LL, Malmberg JA, et al. NK cell responses to cytomegalovirus infection lead to stable imprints in the human KIR repertoire and involve activating KIRs. Blood. 2013; 121(14):2678-2688. (Biology). View Reference
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Giebel S, Nowak I, Wojnar J, et al. Association of KIR2DS4 and its variant KIR1D with leukemia. Leukemia. 2008; 22(11):2129-2130. (Biology). View Reference
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Katz G, Markel G, Mizrahi S, Arnon TI, Mandelboim O. Recognition of HLA-Cw4 but not HLA-Cw6 by the NK cell receptor killer cell Ig-like receptor two-domain short tail number 4. J Immunol. 2001; 166(12):7260-7267. (Biology). View Reference
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Verheyden S, Bernier M, Demanet C. Identification of natural killer cell receptor phenotypes associated with leukemia. Leukemia. 2004; 18(12):2002-2007. (Biology). View Reference
564375 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.