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      Real Yellow/Real Blue Family Campaign

      BD Horizon RealYellow™ and RealBlue™ Reagents

      The superior alternative to PerCP and PE tandem dyes

       

      Reduced Monocyte Background

      Reduced Monocyte 
      Background

      Reduced Spillover

      Reduced Spillover

      Intracellular Staining

      Intracellular Staining

      reduced monocyte background

      RealYellow™ and RealBlue™ Reagents Offer Decreased Monocyte Background Compared to PE-Cy Tandems

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      BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

      RY775-Improved-nonspecific-Binding
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      BD Horizon™ RY775 Reagents provide improved nonspecific binding

      reduced spillover

      RealYellow™ and RealBlue™ Reagents Offer Reduced Spillover Compared to PE and PerCP Tandems

      RB610 Reduced Spillover Compared to PE Dazzle and PE CF594
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      BD Horizon™ RY610 Reagents show reduced spillover into the corresponding blue detectors.

      RB705 Reduced Spillover Compared with PerCP and CY5.5
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      BD Horizon™ RB705 Reagents show reduced spillover into the corresponding yellow-green detectors.

      RY586 Reduced Spillover Compared with PE Tandem Dyes
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      BD Horizon™ RY586 Reagents offer high resolution with minimal cross-laser excitation. Data was acquired on a BDFACSymphony™ A5 SE Cell Analyzer.

      Intracellular marker detection

      RealYellow™ and RealBlue™ Reagents Offer the Detection of Low-expression Surface and Intracellular Markers.

      RY703-Reagent-Lower-Expression-Intracellular-Markers
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      BD Horizon™ RY703 Reagent resolves low-expression intracellular markers

      Cytokine-Marker
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      BD Horizon™ RB780 Reagents support the detection of low-expression intracellular markers.

      RY775-Resolves-Intracellular-Markers
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      BD Horizon™ RY775 Reagent resolves intracellular markers

       

       

      Swap Your Fluorochromes for the Cleaner Versions

      FormatSpectralConventionalRelative BrightnessSpillover
      (1-low, 4-high)      		Alternative To
      Blue Laser
      RB545✓  1(Unique position)
      RB613✓✓ 2PE-CF594, PE-Dazzle™ 594, BB630-P2 or StarBright™ Blue 615
      RB670✓✓ 2PE-Cy5, BB660-P2 or StarBright™ Blue 675
      RB705✓✓ 2PerCP-Cy5.5, BB700 or StarBright™ Blue 700
      RB744✓✓ 1BB755-P
      RB780✓✓ 1PE-Cy7 or BB790-P
      Yellow-Green Laser
      RY586✓✓ 1PE
      RY610✓✓ 1PE-CF594 or PE-Dazzle™ 594
      RY655✓✓ 3PE-Cy5 or StarBright™ Yellow 665
      RY703✓✓ 2PE-Cy5.5 or StarBright™ Yellow 720
      RY743✓✓ 2PE/Fire™ 744
      RY775✓✓ 2PE-Cy7 or StarBright™ Yellow 800
      * On three-laser configuration (B, V, R)
      ** On four- or five-laser configuration (B, V, R, YG or UV, V, B, YG, R)

       

      For more details on these fluorochromes, visit the BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents page.

       

      Request a Sample

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