For Professionals in Clinical Diagnostics
Overview
The BD OneFlow™ PCST is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1*
The BD OneFlow™ PCST is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow as an aid in the diagnosis of hematological disorders.
Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ B-cell Chronic Lymphoproliferative Diseases Tube 1 (B-CLPD T1), BD OneFlow™ Plasma Cell Screening Tube (PCST), BD OneFlow™ Plasma Cell Disorders (PCD) Tube and BD OneFlow™ Acute Leukemia Orientation Tube (ALOT).
The BD OneFlow™ PCST reagent composition
| Antibody | Fluorochrome | Clone | Tube | Target Populations |
|---|---|---|---|---|
| CD38 | FITC | HB7 | S | Backbone markers. Identification of plasma cells |
CD56 | PE | MY31 | S | Identification of aberrant plasma cells |
| β2-Microglobulin | PerCP-Cy5.5 | TU99 | S | Identification of aberrant plasma cells |
| CD19 | PE-Cy7 | SJ25-C1 | S | Backbone markers. Identification of plasma cells. Identify and characterize mature B cells |
| Anti-Kappa | APC | TB28-2 | C | Plasma cells clonality |
| Anti-Lambda | APC-H7 | 1-155-2 | C | Plasma cells clonality |
| CD45 | BD Horizon™ V450 | 2D1 | S | Backbone markers. Identification of plasma cells |
| CD138 | BD Horizon™ V500-C | MI15 | S | Backbone marker. Identification of plasma cells |
A full description of the utility of the antibodies chosen for the BD OneFlow™ PCST is provided in the publication by the EuroFlow Consortium1
APPLICATIONS
Laboratory report of a normal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ PCST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal polyclonal and aberrant plasma cell populations.
Laboratory report of an abnormal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ PCST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal polyclonal and aberrant plasma cell populations.
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Instructions for Use
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Application Guides - For BD FACSLyric™ Flow Cytometer Users
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Applications Guides - For BD FACSCanto™ II Flow Cytometer Users
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Tools
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Application Guides - For BD FACSLyric™ Flow Cytometer Users
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Application Guides - For BD FACSCanto™ II Flow Cytometer Users
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Application Note
References
- van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
- Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
- Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122
The BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers are Class 1 Laser Products.
BD OneFlow™ Reagents, BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ Applications, BD FACSCanto™ II Flow Cytometer, BD™® FC Beads (8-Color, 7-Color, 5-Color and 2-Color Kits), BD OneFlow™ Setup Beads, BD FACSDiva™ CS&T IVD Beads and BD™® CS&T Beads are CE marked in compliance with the European In Vitro Diagnostic Medical Device Directive 98/79/EC.
The EuroFlow trademark is the property of the EuroFlow Consortium and cannot be reproduced or published without prior written permission from the EuroFlow coordinator (www.euroflow.org).
Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
The BD OneFlow™ Solution is intended for professional use only.
*This publication refers to EuroFlow and is relevant to the BD OneFlow™ Solution as the BD OneFlow™ Solution demonstrated equivalent results to EuroFlow through a clinical trial (BD OneFlow™ PCST Instructions for Use).
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
