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BD FACSDiva™ Software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for today's busy laboratory.

BD FACSDiva™ Software provides features to help users integrate flow systems into application areas, such as index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories.


BD FACSDiva™ Software provides robust features from setup through analysis

BD FACSDiva v9.0 Software:

Provides powerful index sorting, along with more routine software operation features including overlays, undo/redo, and multiple copying and pasting of all worksheet elements.

BD FACSDiva™ Software v9.0 and greater run on the Microsoft™ Windows™ 10 64-bit operating system. A selected list of BD FACSDiva™ Software features is listed in the following specification table.

The index sorting feature enables laboratories to sort cells into single wells of plates and also easily identify them on plots, in statistics views and in a detailed results file.

Learn more about Qr and Br in BD FACSDiva™ Software, the parameters for characterizing detector performance.



Index sorting


Feature Specification
Cytometers Supported BD FACSCanto™ 10-color, BD FACSCelesta™, BD FACSCanto™, BD FACSCanto™ II, BD FACSAria™ II, BD FACSAria™ III, BD FACSAria™ Fusion, BD® LSR II, BD LSRFortessa™ and BD LSRFortessa™ X-20
Options Supported BD FACS™ Loader for the BD FACSCanto™ platform, BD® High Throughput Sampler for the BD FACSCanto™ and BD® LSR platforms, and BD FACS™ Accudrop for the BD FACSAria™ platform
Plates Supported U, V and flat-bottom 96-well plates, flat-bottom 384-well plates, Terasaki plates (BD FACSAria™ Cytometer only)
Laser/Filter Support 145 (See Table 1 in Qr and Br in BD FACSDiva™ Software: Parameters for Characterizing Detector Performance)
Event Display User-Defined Event Number, User-Defined Percentage
Standard Gating Interval, Rectangle, Polygon, Quadrants, Hinged Quadrants
Logical Gating And, Or, Not, and Rest Of
Auto Gating Auto-Polygon, Auto-Interval, Snap-To Polygon, Snap-To Interval
Standard Statistics Minimum, Maximum, Geometric Mean, Mean, Median, Standard Deviation, Percent Coefficient of Variation, Mode
Robust Statistics Robust Standard Deviation, Robust Percent Coefficient of Variation
Analysis Types Manual or Batch
Plot Types Histogram, 2D Dot Plot, 2D Contour Plot, 2D Density Plot
Plot Alignment Tools Left, Right, Bottom, Top, Distribute Horizontally, Distribute Vertically
Performance Tracking Measurements PMTV, Qr, Br, Bright Bead Target Value, Bright Bead %rCV, Mid Bead Median, Mid Bead %rCV, Dim Bead Median, Dim Bead %rCV, Forward Scatter Laser Delay, Fluorescence Laser Delay, Laser Current, Laser Power, Fluidics Pressure
Scaling Linear, Log, Biexponential
Automated Setup Define Baseline, Check Performance, Auto Compensation, Reset Target Values
Export Formats JPG, TIFF, PNG, BMP, XML, CSV, PDF
Export Files Experiments, FCS Files, Statistics, Templates
Levey-Jennings Plots Up to 30
Operating System v9.0: Microsoft™ Windows™ 10



BD FACSDiva™ Software supports data integrity and helps ensure reproducible results by enabling automated cytometer setup

Experiment and application setup

The software:

  • Provides convenient and easy experiment layout tools to simplify creation of experiments
  • Enables a graphical view of the entire experiment and allows quick addition of labels, keywords and acquisition attributes to a single tube, multiple tubes, specimens or the entire experiment
  • Offers the ability to store experiment, analysis and panel templates for re-use without sacrificing data integrity
  • Provides the ability to save specific settings to properly resolve populations of interest with the application settings feature. Once a daily performance check is performed to update settings, application settings can be applied to ensure that the particular applications with these settings are running consistently from day to day.

Automated Daily Setup and QC

A single daily setup run using BD FACSDiva™ Software and BD FACSDiva™ CS&T Research Beads* supports consistency and accuracy of data acquisition.


Automatic cytometer adjustments 

Using the Experiment Layout feature in BD FACSDiva™ Software greatly simplifies and speeds up creating a new experiment.

Key cytometer setup values for PMT voltages, laser delay and area scaling factors are automatically calculated and adjusted to maintain optimal performance levels over time. This automation reduces startup time to approximately five minutes and eliminates multiple error-prone and expensive data acquisitions and calculations.


Note that because of a change in the beads for BD FACSDiva™ Software v7.0 and v8.0, upgrading from BD FACSDiva™ Software v6.x to v8.0 will cause a loss of existing baselines and application settings.


All baselines, performance checks and application settings will have to be recreated, as needed. See the BD FACSDiva Software Reference Manual and the BD Cytometer Setup and Tracking Application Guide for more details.


Cytometer Configuration Setup

BD FACSDiva™ Software provides a graphic of the cytometer's physical optical configuration, showing the layout of detectors, filters and mirrors. This allows users to select the correct configuration for their work.


Administrators can create specialized configurations for particular applications by dragging filters, mirrors and fluorochromes onto a representation of the optical bench.


Visual tools for cytometer configuration setup

Cytometer configuration setup is quickly completed through drag and drop of parameters, filters or mirrors onto the appropriate optical bench location.

BD FACSDiva™ Software provides automated performance tracking and simplifies quality control

The software automatically tracks a wide range of performance metrics for each detector and laser in the system and displays the results in Levey-Jennings charts. 


Alarm criteria can also be set to automatically notify users if performance metrics fall outside these boundaries.


BD FACSDiva™ Software enables efficient organization of steps

Efficient and accurate experiment setup

BD FACSDiva™ Software helps users manage experimental details with easy-to-use interfaces and controls.



BD FACSDiva™ Software simplifies data acquisition with a dashboard that provides the tools for immediately viewing acquisition status and for setting acquisition stopping criteria. The application settings feature automatically adjusts voltage settings to daily changes in cytometer performance so that users can run existing applications more simply and quickly.


BD FACSDiva™ Software provides the ability to overlay multiple different samples and any or all populations within each tube of data.


BD FACSDiva™ Software provides a wide array of basic analysis features enabling multiple applications to run on the BD FACSCanto™, BD® LSR and BD FACSAria™ Cytometers. These include gating tools to automatically find a population, biexponential scaling to completely see populations that might have negative relative fluorescence intensities, and even overlays to compare multiple tubes or samples.



Worksheets are configurable using predefined and customizable headers and footers. Snap-to grids allow for easy alignments of plots, comments and statistics. Previewing and PDF creation can be completed within the BD FACSDiva™ Software interface.


Data management

BD FACSDiva™ Software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. Along with built-in database and database utilities, BD FACSDiva™ Software provides automated data export in a variety of formats including XML, FCS and even ZIP, for multiple files concurrently. Manual export of FCS files creates an experiment folder structure in the operating system for easy identification of data files without the need to open them.


BD FACSDiva™ Software offers simple tools for data reporting that provide flexible defined layout definiton and easy previewing and printing.


Proactive Performance Monitoring

On a daily basis, BD FACSDiva™ Software automates tracking of all performance metrics, including detection efficiency, standard deviation of electronic noise, laser current and optical background. Viewing these metrics in interactive Levey-Jennings charts, administrators can visually adjust displays and time scales. The software allows notification when performance metrics fall outside specified boundaries for early troubleshooting and correction.






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For Professionals in Research and Clinical Diagnostics


Microsoft, Windows and Excel are trademarks of Microsoft Corporation.

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation.

CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

*The BD® Cytometer Setup and Tracking application and BD FACSDiva™ CS&T Research Beads are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.