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BD Pharmingen™ Purified Rat Anti-Mouse CD138
Clone 281-2 (RUO)
Expression of CD138 on mouse bone- marrow B lymphocytes. BALB/c bone- marrow leukocytes were stained with purified 281-2 mAb (Right panel), followed by biotinylated goat anti-rat Ig (Cat. no. 554014, both panels), then blocked with normal rat serum (both panels) and stained with Streptavidin-PE (Cat. no. 554061, both panels) and FITCconjugated RA3-6B2 mAb (anti-mouse CD45R/B220, Cat. no. 553087/553088, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Expression of CD138 on mouse bone- marrow B lymphocytes. BALB/c bone- marrow leukocytes were stained with purified 281-2 mAb (Right panel), followed by biotinylated goat anti-rat Ig (Cat. no. 554014, both panels), then blocked with normal rat serum (both panels) and stained with Streptavidin-PE (Cat. no. 554061, both panels) and FITCconjugated RA3-6B2 mAb (anti-mouse CD45R/B220, Cat. no. 553087/553088, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Expression of CD138 on mouse bone- marrow B lymphocytes. BALB/c bone- marrow leukocytes were stained with purified 281-2 mAb (Right panel), followed by biotinylated goat anti-rat Ig (Cat. no. 554014, both panels), then blocked with normal rat serum (both panels) and stained with Streptavidin-PE (Cat. no. 554061, both panels) and FITCconjugated RA3-6B2 mAb (anti-mouse CD45R/B220, Cat. no. 553087/553088, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Expression of CD138 on mouse bone- marrow B lymphocytes. BALB/c bone- marrow leukocytes were stained with purified 281-2 mAb (Right panel), followed by biotinylated goat anti-rat Ig (Cat. no. 554014, both panels), then blocked with normal rat serum (both panels) and stained with Streptavidin-PE (Cat. no. 554061, both panels) and FITCconjugated RA3-6B2 mAb (anti-mouse CD45R/B220, Cat. no. 553087/553088, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Product Details
BD Pharmingen™
SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
NAMRU mouse mammary gland epithelial cell line NMuMG
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), ELISA, Immunohistochemistry-paraffin, Western blot (Reported)
0.5 mg/ml
AB_394998
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
CD138 is expressed at low density on cells in the normal bone marrow; therefore, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. no. 554061). Other reported applications include western blot analysis using a cationic blotting membrane and a specific method, ELISA, and immunohistochemical staining of acetone-fixed frozen and paraffin-embedded sections.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Purified Rat IgG2a, κ Isotype Control RUO
Size
0.5 mg
Cat No.
553927
Biotin Goat Anti-Rat Ig RUO
Size
0.5 mg
Cat No.
554014
FITC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553087
PE Streptavidin RUO
Size
0.5 mg
Cat No.
554061
553712 Rev. 9
Antibody Details
281-2
The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow, and antibody-secreting cells including plasma cells (but not mature peripheral B cells). It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.
This antibody is routinely tested by flow cytometric analysis. Other application were tested at BD Biosciences Pharmingen during development only or reported in the litererature.
553712 Rev. 9
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553712 Rev.9
Citations & References
View product citations for antibody "553712" on CiteAb
Development References (9)
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Bernfield M, Kokenyesi R, Kato M, et al. Biology of the syndecans: a family of transmembrane heparan sulfate proteoglycans. Annu Rev Cell Biol. 1992; 8:365-393. (Biology). View Reference
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Driver DJ, McHeyzer-Williams LJ, Cool M, Stetson DB, McHeyzer-Williams MG. Development and maintenance of a B220- memory B cell compartment. J Immunol. 2001; 167(3):1393-1405. (Biology). View Reference
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Fitzgerald ML, Wang Z, Park PW, Murphy G, Bernfield M. Shedding of syndecan-1 and -4 ectodomains is regulated by multiple signaling pathways and mediated by a TIMP-3-sensitive metalloproteinase. J Cell Biol. 2000; 148(4):811-824. (Biology). View Reference
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Hayashi K, Hayashi M, Jalkanen M, Firestone JH, Trelstad RL, Bernfield M. Immunocytochemistry of cell surface heparan sulfate proteoglycan in mouse tissues. A light and electron microscopic study. J Histochem Cytochem. 1987; 35(10):1079-1088. (Clone-specific: Immunohistochemistry). View Reference
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Jalkanen M, Nguyen H, Rapraeger A, Kurn N, Bernfield M. Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody. J Cell Biol. 1985; 101(3):976-984. (Clone-specific: ELISA, Western blot). View Reference
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Lalor PA, Nossal GJ, Sanderson RD, McHeyzer-Williams MG. Functional and molecular characterization of single, (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific, IgG1+ B cells from antibody-secreting and memory B cell pathways in the C57BL/6 immune response to NP. Eur J Immunol. 1992; 22(11):3001-3011. (Biology: Western blot). View Reference
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Sanderson RD, Lalor P, Bernfield M. B lymphocytes express and lose syndecan at specific stages of differentiation. Cell Regul. 1989; 1(1):27-35. (Clone-specific: Western blot). View Reference
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Sanderson RD, Sneed TB, Young LA, Sullivan GL, Lander AD. Adhesion of B lymphoid (MPC-11) cells to type I collagen is mediated by integral membrane proteoglycan, syndecan. J Immunol. 1992; 148(12):3902-3911. (Biology). View Reference
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Saunders S, Jalkanen M, O'Farrell S, Bernfield M. Molecular cloning of syndecan, an integral membrane proteoglycan. J Cell Biol. 1989; 108(4):1547-1556. (Biology). View Reference
553712 Rev. 9
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.