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Purified Mouse anti-TBK1 (pS172)
Purified Mouse anti-TBK1 (pS172)
Western blot analysis of TBK1 (pS172) fusion protein.  Lysates from control 293 fetal kidney cell line (left panel) and transfected 293T/IRF-7/TBK-1 cells (right panel) were probed with purified mouse anti-TBK1 (pS172) monoclonal antibody at concentrations of 0.0312 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6).  TBK1-GFP fusion protein is identified as a band of 115 kDa in the transfected cells, which can be completely removed by treatment with lambda protein phosphatase (data not shown).  The molecular weight of native human TBK1 protein has been reported to be 80 kDa.
Western blot analysis of TBK1 (pS172) fusion protein.  Lysates from control 293 fetal kidney cell line (left panel) and transfected 293T/IRF-7/TBK-1 cells (right panel) were probed with purified mouse anti-TBK1 (pS172) monoclonal antibody at concentrations of 0.0312 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6).  TBK1-GFP fusion protein is identified as a band of 115 kDa in the transfected cells, which can be completely removed by treatment with lambda protein phosphatase (data not shown).  The molecular weight of native human TBK1 protein has been reported to be 80 kDa.
Product Details
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BD Pharmingen™
T2K, NAK
Human (QC Testing), Mouse (Predicted)
Mouse IgG2b, κ
Phosphorylated Human TBK1
Western blot (Routinely Tested)
80 kDa
0.5 mg/ml
AB_647277
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558397 Rev. 1
Antibody Details
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J133-1171

NF-κB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes.  It is involved in immune, inflammatory, viral, and acute phase responses.  In most cells, NF-κB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IκBα, IκBβ, and IκBε.  Stimulation induces the release, activation, and nuclear translocation of NF-κB.  Release of NF-κB results from the phosphorylation and proteolytic degradation of the IκB proteins.  Two cytokine-inducible IκB kinases (IKKα and IKKβ) phosphorylate and target the IκB proteins for degradation via the ubiquitin pathway.  IKKγ/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKβ.  TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-κB downstream of the tumor necrosis factor and Toll-like receptor pathways.  TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-κB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172).

The J133-1171 monoclonal antibody recognizes the phosphorylated S172 of human TBK1.

558397 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558397 Rev.1
Citations & References
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View product citations for antibody "558397" on CiteAb

Development References (2)

  1. Kishore N, Huynh QK, Mathialagan S, et al. IKK-i and TBK-1 are enzymatically distinct from the homologous enzyme IKK-2: comparative analysis of recombinant human IKK-i, TBK-1, and IKK-2.. J Biol Chem. 2002; 277(16):13840-13847. (Biology). View Reference
  2. Viatour P, Merville M-P, Bours V, Chariot A. Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation. Trends Biochem Sci. 2005; 30(1):43-52. (Biology). View Reference
558397 Rev. 1

 

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.