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DAB Substrate Kit

BD Pharmingen™ DAB Substrate Kit

Product Details
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BD Pharmingen™
Immunohistochemistry-frozen, Immunohistochemistry-paraffin (Routinely Tested)


3,3' Diaminobenzidine (DAB) is a substrate for the enzyme peroxidase and is a widely used chromogen for immunohistochemical staining and immunoblotting. In the presence of peroxidase, DAB produces a brown precipitate that is insoluble in alcohol.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Immunohistochemistry:  Just before staining, add one drop (~ 40 µL) of DAB Chromogen for every 1 mL of DAB Buffer.  The mixed DAB substrate solution can be stored for up to 6 hours, making it ideal for use in automated stainers.  Storing the reagent for longer periods of time will cause precipitate formation.  Apply the mixed DAB substrate reagent to the tissue section after it has been incubated with the appropriate horseradish peroxidase (HRP) conjugated reagents and washing is complete.  Approximately 100 µL of the mixed DAB substrate reagent should be sufficient to cover the entire tissue section.   Incubate the section with the mixed DAB reagent for 5-15 minutes or until the desired color intensity is obtained.

DAB Buffer (component 51-7548KC) is a 50 mL solution of citric acid and disodium hydrogenorthophosphate.

Danger:  DAB Chromogen (component 51-7549KC) is a 2.5 mL solution containing 1.74% 3,3' diaminobenzidine (w/w).

Hazard statements:                                        Precautionary statements:

Suspected of causing genetic defects.                Wear protective clothing / eye protection.

May cause cancer.                                                Wear protective gloves.

                                                                        Do not handle until all safety precautions have been read and understood.

                                                                        IF exposed or concerned: Get medical advice/attention.

                                                                        Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

  1. Please refer to for technical protocols.
  2. Please refer to to access safety data sheets (SDS).
550880 Rev. 4
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Description Quantity/Size Part Number EntrezGene ID
DAB Buffer (50 ml) N/A 51-7548KC N/A
DAB Chromogen (2.5 ml) N/A 51-7549KC N/A
550880 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.