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Purified Mouse Anti-Rabaptin-5
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human Rabaptin-5 aa. 247-417.
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
117 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610676 Rev. 1
Antibody Details
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Rabs are small GTP-binding proteins that are localized to specific intracellular vesicles and organelles. They are important for the regulation of vesicular traffic. It is thought that Rabs cycle between GTP- and GDP-bound forms and this cycle is related to their function. Rabaptin-5 is protein that interacts with GTP-Rab5. Although Rabaptin-5 is localized in the cytosol, it is also recruited to the endosomal fraction in a Rab5 and GTP-dependent manner. The removal of Rabaptin-5 from the cytosol substantially impairs GTP and Rab5-dependent endosomal fusion. This indicates that Rabaptin-5 may be an important partner of Rab5 during endocytosis. Rabaptin-5 contains 862 amino acids and both ends of the protein contain coiled-coil domains typically found in vesicular transport proteins.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (3)

  1. Shiba Y, Takatsu H, Shin HW, Nakayama K. Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain. J Biochem (Tokyo). 2002; 131(3):327-336. (Biology: Western blot). View Reference
  2. Stenmark H, Vitale G, Ullrich O, Zerial M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995; 83(3):423-432. (Biology). View Reference
  3. Zhu Y, Doray B, Poussu A, Lehto VP, Kornfeld S. Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor. Science. 2001; 292(5522):1716-1718. (Biology: Immunofluorescence, Immunoprecipitation). View Reference
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.