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BD Transduction Laboratories™ Purified Mouse Anti-Human Nek2
Clone 20/Nek2 (RUO)
Western blot analysis of Nek2 on a Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human Nek2 antibody.
Immunofluorescence staining of rabbit kidney.
Western blot analysis of Nek2 on a Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human Nek2 antibody.
Immunofluorescence staining of rabbit kidney.
Product Details
BD Transduction Laboratories™
Nima Related Kinase 2
Human (QC Testing)
Mouse IgG1
Human Nek2 aa. 244-444
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunoprecipitation (Not Recommended)
46 kDa
250 µg/ml
AB_397934
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Jurkat Cell Lysate RUO
Size
500 µg
Cat No.
611451
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
610594 Rev. 1
Antibody Details
20/Nek2
Reversible protein phosphorylation is critical for progression through the cell cycle and mitosis. In Aspergillus nidulans, the nima gene (never in mitosis) encodes a protein kinase that is essential for mitosis. Three human genes (nek1, 2, and 3 [Nima-related kinase]) with significant homology to the A. nidulans nima have been reported. The nek2 gene encodes a protein of 445 amino acids and, like its fungal homolog, its expression is regulated throughout the cell cycle. In Hela cells, Nek2 activity and expression are low during M and G1 phases of the cell cycle. However, both parameters increase during S phase and mitosis. In addition, Nek2 phosphorylates protein substrates exclusively at serine and threonine residues. Thus, like its fungal homolog, Nek2 may be a crucial element in controlling the cell's entry into S phase and mitosis.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
610594 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610594 Rev.1
Citations & References
View product citations for antibody "610594" on CiteAb
Development References (2)
-
Fry AM, Schultz SJ, Bartek J, Nigg EA. Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans. J Biol Chem. 1995; 270(21):12899-12905. (Biology). View Reference
-
Schultz SJ, Fry AM, Sutterlin C, Ried T, Nigg EA. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. Cell Growth Differ. 1994; 5(6):625-635. (Biology). View Reference
610594 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.