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Purified Mouse Anti-Human Ku80
Purified Mouse Anti-Human Ku80

Western blot analysis of Ku80 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human Ku80 antibody.

Purified Mouse Anti-Human Ku80

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of Ku80 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human Ku80 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Ku80 aa. 103-315
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
80 kDa
250 µg/ml
AB_398882
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611360 Rev. 1
Antibody Details
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7/Ku80

DNA-dependent protein kinase (DNA-PK) is a trimeric enzyme that contains a catalytic subunit of 350 kDa (DNA-PKcs) and a heterodimeric regulatory subunit of 70 kDa (Ku70) and 86 kDa (Ku80). DNA-PKcs is inactive alone and depends on the regulatory subunit for subcellular localization and kinase activity. The DNA-PKcs/Ku70/Ku80 complex is involved in V(D)J recombination and DNA double-stranded break repair. Ku70 and Ku80 are abundant nuclear DNA-binding proteins. Besides functioning in a complex with DNA-PKcs, Ku proteins may act in multiple cellular processes including transcriptional regulation, ATPase and helicase activity, alteration in chromatin structure, cell cycle regulation, and maintenance of telomere length. In rat fibroblasts, Ku80 overexpression leads to hypermethylation and silencing of metallothionein gene expression. Ku70-/- and Ku80-/- cells are less resistant to anticancer drug-induced apoptosis, suggesting a role for Ku proteins in the prevention of apoptotic signaling. Thus, Ku70 and Ku80 may have multiple roles during DNA transcription, repair, and maintenance depending on the protein interactions that are involved.

611360 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611360 Rev.1
Citations & References
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Development References (3)

  1. Kim SH, Kim D, Han JS, et al. Ku autoantigen affects the susceptibility to anticancer drugs. Cancer Res. 1999; 59(16):4012-4017. (Biology). View Reference
  2. Majumder S, Ghoshal K, Li Z, Jacob ST. Hypermethylation of metallothionein-I promoter and suppression of its induction in cell lines overexpressing the large subunit of Ku protein. J Biol Chem. 1999; 274(40):28584-28589. (Biology). View Reference
  3. Smith GC, Jackson SP. The DNA-dependent protein kinase.. Genes Dev. 1999; 13(8):916-34. (Biology). View Reference
611360 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.