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Purified Mouse Anti-CaM Kinase Kinase
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Dog (Tested in Development)
Mouse IgG2a
Rat CaM Kinase Kinase aa. 341-504
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
68 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Antibody Details
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6/CaM Kinase Kinase

Ca2+/Calmodulin (CaM)-dependent kinases are multifunctional kinases involved in a myriad of cellular functions such as neurotransmitter synthesis, long-term potentiation, and formation of spatial learning. A 68kDa CaM Kinase Kinase with homology to the Ca2+/CaM-dependent kinases, phosphorylates and activates Ca2+/CaM-dependent protein kinases I and IV, but not type II. This phosphorylation occurs on Ser/Thr residues and is Ca2+ and CaM dependent. CaM Kinase Kinase is abundant in brain tissue, and is thought to be an upstream regulator in the Ca2+/CaM-dependent neural processes.    

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610544 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (5)

  1. Edelman AM, Mitchelhill KI, Selbert MA. Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca(2+)-calmodulin, and partial amino acid sequence. J Biol Chem. 1996; 271(18):10806-10810. (Biology). View Reference
  2. Egea J, Espinet C, Soler RM. Neuronal survival induced by neurotrophins requires calmodulin. J Cell Biol. 2001; 154(3):585-597. (Clone-specific: Western blot). View Reference
  3. Tokumitsu H, Enslen H, Soderling TR. Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase. J Biol Chem. 1995; 270(33):19320-19324. (Biology). View Reference
  4. Wayman GA, Walters MJ, Kolibaba K, Soderling TR, Christian JL. CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner. J Cell Biol. 2000; 151(4):811-824. (Clone-specific: Western blot). View Reference
  5. Wu JY, Means AR. Ca(2+)/calmodulin-dependent protein kinase IV is expressed in spermatids and targeted to chromatin and the nuclear matrix. J Biol Chem. 2000; 275(11):7994-7999. (Clone-specific: Western blot). View Reference
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.