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Purified Mouse Anti-5-Lipoxygenase
Product Details
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BD Transduction Laboratories™
Chicken (QC Testing), Human, Mouse, Rat (Tested in Development)
Mouse IgG1
Human 5-Lipoxygenase aa. 442-590
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
79 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610695 Rev. 1
Antibody Details
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5-Lipoxygenase (5-LO) is the initial enzyme that converts arachidonic acid to leukotrienes, which are important inflammatory and vasoconstrictive metabolites.  It is activated in response to a number of stimuli, such as differentiation and allergen challenges. The 5-LO gene, abundantly expressed in placenta, lung, and leukocytes, encodes a protein of 674 amino acids with an apparent molecular weight of 78kDa. 5-LO is a Ca2+ and ATP-dependent enzyme that translocates from the cytosol to either a nuclear or plasma membrane compartment following activation. A proline-rich domain of 5-LO (amino acids 566-577) has been identified as a binding site for the PTyr-binding protein, Grb2. This Grb2 site links tyrosine kinases with activation and redistribution of 5-LO. Furthermore, tyrosine kinase inhibitors increase the activity of 5-LO and block the enzyme's subcellular redistribution.

610695 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610695 Rev.1
Citations & References
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Development References (5)

  1. Hundley TR, Prasad AR, Beaven MA. Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase. J Immunol. 2001; 167(3):1629-1636. (Clone-specific: Western blot). View Reference
  2. Lepley RA, Fitzpatrick FA. 5-Lipoxygenase contains a functional Src homology 3-binding motif that interacts with the Src homology 3 domain of Grb2 and cytoskeletal proteins. J Biol Chem. 1994; 269(39):24163-24168. (Biology). View Reference
  3. Lepley RA, Muskardin DT, Fitzpatrick FA. Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase. J Biol Chem. 1996; 271(11):6179-6184. (Biology). View Reference
  4. Matsumoto T, Funk CD, Radmark O, Hoog JO, Jornvall H, Samuelsson B. Molecular cloning and amino acid sequence of human 5-lipoxygenase. Proc Natl Acad Sci U S A. 1988; 85(1):26-30. (Biology). View Reference
  5. Zaitsu M, Hamasaki Y, Matsuo M. New induction of leukotriene A(4) hydrolase by interleukin-4 and interleukin-13 in human polymorphonuclear leukocytes. Blood. 2000 ; 96(2):601-609. (Clone-specific: Western blot). View Reference
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610695 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.