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      Purified NA/LE Rat Anti-Mouse IFN-γ
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG1, κ
      Partially-Purified Mouse IFN-γ
      ELISA (Routinely Tested), Neutralization (Tested During Development)
      1.0 mg/ml
      15978
      AB_395387
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      ELISA:  Purified Rat Anti-Mouse IFN-γ (Clone R4-6A2, Cat. No. 551216) has been reported to be useful as a capture antibody for sandwich ELISA measuring mouse IFN-γ protein levels. Purified R4-6A2 antibody can be paired with Biotin Rat Anti-Mouse IFN-γ (XMG1.2, Cat. No. 554410) as the detection antibody and using recombinant mouse IFN-γ (Cat. No. 554587) as the standard.  For measuring mouse IFN-γ in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IFN-γ ELISA Set (Cat. No. 551866) or BD OptEIA™ Mouse IFN-γ ELISA Kit II (Cat No. 558258).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554430 Rev. 2
      Antibody Details
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      R4-6A2

      The R4-6A2 antibody reacts with mouse interferon-γ (IFN-γ). The immunogen used to generate the R4-6A2 hybridoma was partially purified mouse IFN-γ protein. This is a neutralizing antibody.  

      554430 Rev. 2
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554430 Rev.2
      Citations & References
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      Development References (5)

      1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology: ELISA). View Reference
      2. Mo XY, Sarawar SR, Doherty PC. Induction of cytokines in mice with parainfluenza pneumonia. J Virol. 1995; 69(2):1288-1291. (Biology: ELISA). View Reference
      3. Sarawar SR, Sangster M, Coffman RL, Doherty PC. Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype. J Immunol. 1994; 153(3):1246-1253. (Biology: ELISA). View Reference
      4. Spitalny GL, Havell EA. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J Exp Med. 1984; 159(5):1560-1565. (Immunogen). View Reference
      5. Yang X, HayGlass KT. A simple, sensitive, dual mAb based ELISA for murine gamma interferon determination: comparison with two common bioassays. J Immunoassay. 1993; 14(3):129-148. (Biology: ELISA). View Reference
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      554430 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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