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Purified NA/LE Mouse Anti-Human IFN-γ
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Recombinant human IFN-γ
ELISA (Routinely Tested), Neutralization (Tested During Development)
1.0 mg/ml
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

ELISA:  Purified Mouse Anti-Human IFN-γ antibody (Clone NIB42, Cat. No 551221) can be useful as a capture antibody in sandwich ELISA for measuring human IFN-γ protein levels. Purified NIB42 antibody can be paired with Biotin Mouse Anti-Human IFN-γ mAb, (Clone 4S.B3, Cat. No. 554550) as the detection antibody along with recombinant human IFN-γ (Cat. No. 554617) as the standard.  For measuring human IFN-γ in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IFN-γ ELISA Set (Cat. No. 555142) or  BD OptEIA™ Human IFN-γ ELISA Kit II (Cat. No. 550612).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
554547 Rev. 2
Antibody Details
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The NIB42 antibody reacts with human interferon-gamma (IFN-γ). The immunogen used to generate the NIB42 hybridoma was recombinant human IFN-γ. This is a neutralizing antibody.

554547 Rev. 2
Format Details
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NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
554547 Rev.2
Citations & References
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View product citations for antibody "554547" on CiteAb

Development References (2)

  1. Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Biology). View Reference
  2. Meager A. Characterization of interferons and immunoassays. In: Clemens MJ, Morris AG, Gearing AJH, ed. Lymphokines and Interferons. A Practical Approach. Oxford: IRL Press Ltd; 1987:105-127.
554547 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.