Description

The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich enzyme-linked immunosorbent assay (ELISA), the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of analyte-producing cells (eg, 1/300,000). Recent developments in assay plate design and in ELISPOT plate-reader instrumentation have significantly improved the utility of the ELISPOT method for objective and rapid analysis of analyte-producing cells.

Contents
10 ELISPOT plates
Unlabeled Capture
Antibody Biotinylated Detection
Antibody Enzyme Conjugate
Certificate of Analysis

551491 Rev. 1

Citations & References

Development References (10)

Czerkinsky C, Andersson G, Ekre HP, Nilsson LA, Klareskog L, Ouchterlony O. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells. J Immunol Methods. 1988; 110(1):29-36. (Biology). View Reference
Czerkinsky CC, Nilsson LA, Nygren H, Ouchterlony O, Tarkowski A. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods. 1983; 65(1-2):109-121. (Biology). View Reference
Czerkinsky, CC, LA Nilsson, H Nygren, O Ouchterlony, and A Tarkowski. A solid phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibodysecreting cells. J Immunol Meth. 1983; 65:109. (Biology).
Fujihashi, K, J McGhee, et al. Cytokine-specific ELISPOT assay: single cell analysis of IL-2, IL-4, and IL-6 producing cells. J Immunol Meth. 1993; 160:181-189. (Biology).
Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J Immunol. 2000; 164(7):3723-3732. (Biology). View Reference
Power, C, C Grand, et al. A valid ELISPOT assay for enumeration of ex vivo antigen-specific IFN-γ producing T cells. J Immunol Meth. 1999; 227:99-107. (Biology).
Ronnblom L, Cederblad B, Sandberg K, Alm GV. Determination of herpes simplex virus-induced alpha interferon-secreting human blood leucocytes by a filter immuno-plaque assay. Scand J Immunol. 1988; 27(2):165-170. (Biology). View Reference
Tary-Lehmann, M, D Hricik, A Justice, N Potter, and P Heeger. Enzyme-linked immunosorbent assay spot detection of interferon-γ and interleukin 5-producing cells as a predictive marker for renal allograft failure. Transplantation. 1998; 66:219-224. (Biology).
VanCott, J, H Staats, et al. Regulation of mucosal and systematic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella. J Immunol. 1996; 156:1504-1514. (Biology).
Yip, H, A Karulin, et al. Adjuvant-guided type-1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response. J Immunol. 1999; 162:3942-3949. (Biology).

View All (10)

551491 Rev. 1

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