Purified Mouse Anti-Pig IFN-γ

BD Pharmingen™ Purified Mouse Anti-Pig IFN-γ

Name: Purified Mouse Anti-Pig IFN-γ
Brand: BD Pharmingen™
SKU: 559961

Clone P2G10

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Pig (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Recombinant pig IFN-γ protein

Application: ELISA Capture (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_397397

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified P2G10 antibody (Cat. No. 559961) is useful as a capture antibody in a sandwich ELISA for measuring pig IFN-γ protein levels. Purified P2G10 antibody can be paired with the biotinylated P2C11 antibody (Cat. No.559958) as the detecting antibody. Purified P2G10 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of pig IFN-γ ranging from ~500 to 4 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols section or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

Immunofluorescence and Flow cytometry: For immunofluorescent staining and flow cytometric analysis, the P2G10 antibody has been found useful to identify and enumerate IFN-γ producing cells within mixed cell populations. PE- conjugated P2G10 antibody (Cat. No. 559812) is especially suitable for these studies. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

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Antibody Details

P2G10

The P2G10 monoclonal antibody specifically binds to porcine interferon-γ (IFN-γ). The immunogen used to generate the P2G10 hybridoma was recombinant pig IFN-γ protein.

559961 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

559961 Rev.1

Citations & References

Development References (2)

Mateu de Antonio E, Husmann RJ, Hansen R, et al. Quantitative detection of porcine interferon-gamma in response to mitogen, superantigen and recall viral antigen. Vet Immunol Immunopathol. 1998; 61(2-4):265-277. (Immunogen: ELISA). View Reference
Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). View Reference

559961 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.