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      HRP Rat Anti-Mouse IgM
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG2a, κ
      Not reported
      ELISA Detection (Routinely Tested)
      AB_393767
      Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
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      Recommended Assay Procedures

      ELISA: For detection of mouse IgM by sandwich ELISA, this antibody may be used as the detection antibody at a ~1:1000 dilution coupled with purified rat anti-mouse IgM antibody (clone R6-60.2) (Cat.No. 553405) as the capture antibody.  Purified mouse IgM (Cat.No. 553472) may be used as the ELISA standard.

      Warning:  HRP Rat Anti-Mouse IgM contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

      Hazard statements

      May cause an allergic skin reaction.

      Precautionary statements

      Wear protective gloves / eye protection.

      Wear protective clothing.

      Avoid breathing mist/vapours/spray.

      If skin irritation or rash occurs: Get medical advice/attention.

      IF ON SKIN: Wash with plenty of water.

      Dispose of contents/container in accordance with local/regional/national/international regulations.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. ProClin is a trademark of Rohm and Haas Company.
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      550588 Rev. 8
      Antibody Details
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      II/41

      The II/41 clone has been reported to react specifically with mouse IgM of Igh-C[a] and Igh-C[b] haplotypes. It has been reported not to react with other Ig isotypes. In addition, the II/41 clone has been reported not to stimulate B-cell proliferation.

      550588 Rev. 8
      Format Details
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      HRP
      Horseradish Peroxidase (HRP) is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide resulting in a visible colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with HRP and used in applications such as i) ELISA that uses a plate reader to measure absorbance of the colored product, or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
      HRP
      550588 Rev.8
      Citations & References
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      View product citations for antibody "550588" on CiteAb

      Development References (1)

      1. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Biology). View Reference
      550588 Rev. 8

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.