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AKP Streptavidin
Product Details
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BD Pharmingen™
ELISA (Routinely Tested)
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed

For extended storage, we recommend the addition of an equal volume of glycerol, making a final concentration of 50% glycerol and reducing the concentration by 50%.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Please refer to to access safety data sheets (SDS).
  6. Please refer to for technical protocols.
554065 Rev. 16
Antibody Details
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This reagent conjugate can be used as a detecting antibody in ELISA; a dilution of 1:1000 is recommended (1:500 if glycerol has been added). For immunohistochemical staining, we recommend the use of SAv-AKP in our special formulation for immunohistochemistry, Cat. No. 551008. SAv-AKP is a useful second-step reagent for labeling a biotinylated primary antibody for detection by ELISA (with an appropriate substrate system).

554065 Rev. 16
Format Details
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Alkaline phosphatase is an enzyme that is capable of cleaving various substrates, usually producing a detectable product. Antibodies, avidins, and other specific binding molecules conjugated with alkaline phosphatase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
554065 Rev.16
Citations & References
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View product citations for antibody "554065" on CiteAb
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554065 Rev. 16

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.