Mouse Enhanced Sensitivity Master Buffer Kit

BD™ Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit

Name: Mouse Enhanced Sensitivity Master Buffer Kit
Brand: Mouse Enhanced Sensitivity Master Buffer Kit
SKU: 562248

Protocol Library

Scientific Resources

Protocol Library

Scientific Resources

Product Details

Brand: BD™ Cytometric Bead Array (CBA)

Reactivity: Mouse (QC Testing)

Application: Flow cytometry (Routinely Tested)

Regulatory Status: RUO

RRID: AB_2869406

Description

The BD™ CBA Human IL-1β Flex Set is a bead-based immunoassay capable of measuring human interleukin-1β (IL-1β) in serum, plasma, and cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human IL-1β Flex Set. The biology and function of IL-1β has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).

Preparation And Storage

Store all kit components at 2 to 8°C. Do not freeze.

This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Human IL-1β Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-1β Flex Set will be 2,500 pg/ml. An example standard curve is shown in Figure 1.

The BD CBA Human IL-1β Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset.

Performance

Limit of Detection: The theoretical limit of detection is 2.3 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.

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Components

Description	Quantity/Size	Part Number	EntrezGene ID
Assay Diluent	100 Tests (1 ea)	51-9003992	N/A
Capture Bead Diluent	100 Tests (1 ea)	51-9003804	N/A
Detection Reagent Diluent A	15 mL (1 ea)	51-9007667	N/A
Detection Reagent Diluent B	35 mL (2 ea)	51-9007668	N/A
Wash Buffer	100 Tests (2 ea)	51-9003798	N/A
Instrument Setup Bead A1	N/A	51-9003855	N/A
Instrument Setup Bead A9	N/A	51-9003858	N/A
Instrument Setup Bead F1	100 Tests (1 ea)	51-9003851	N/A
Instrument Setup Bead F9	N/A	51-9003854	N/A
PE Instrument Setup Bead F1	100 Tests (1 ea)	51-9005038	N/A
PE* Positive Control Detector	100 Tests (1 ea)	51-9005065	N/A
Enhanced Sensitivity Detection Reagent	N/A	51-9007218	N/A

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Citations & References

Development References (10)

Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). View Reference
McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). View Reference
Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).

View All (10)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.