Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
France (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Rat CD3
      Purified Mouse Anti-Rat CD3
      CD3e expression in spleen and thymus. LEW splenocytes were preincubated with Rat BD Fc Block™ purified anti-rat CD32 mAb D34-485 (Cat. No. 550270/550271) and simultaneously stained with FITC-onjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449, left panels) and purified mAb 1F4 (middle left panel), followed by PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553517, left panels). LEW thymocytes were stained with purified 1F4 mAb (far right panel) followed by PE-conjugated anti-mouse IgMa mAb DS-1 (right panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Purified Mouse Anti-Rat CD3
      CD3e expression in spleen and thymus. LEW splenocytes were preincubated with Rat BD Fc Block™ purified anti-rat CD32 mAb D34-485 (Cat. No. 550270/550271) and simultaneously stained with FITC-onjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449, left panels) and purified mAb 1F4 (middle left panel), followed by PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553517, left panels). LEW thymocytes were stained with purified 1F4 mAb (far right panel) followed by PE-conjugated anti-mouse IgMa mAb DS-1 (right panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Rat (QC Testing)
      Mouse BALB/c IgM, κ
      F344 rat splenic T cells stimulated with PMA plus A23187
      Flow cytometry (Routinely Tested), Cytotoxicity, Fluorescence microscopy, Immunohistochemistry-frozen, Immunoprecipitation, Stimulation (Reported)
      0.5 mg/ml
      AB_396542
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      We recommend FITC- or PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553516 or 553517, respectively) as a secondary antibody for immunofluorescent staining because we have found that polyclonal anti-mouse Ig antibodies may lose much of their reactivity to mouse IgM after adsorption with rat Ig. For IHC, we recommend the use of purified G4.18 mAb in our special formulation for immunohistochemistry, Cat. No. 550295. Although mAb 1F4 is reported to be effective for in vitro T-cell stimulation, we have found that the alternate anti-rat CD3 antibody G4.18 (Cat. no. 554829), soluble or immobilized, has greater stimulatory activity.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      Show More blue down arrow Show Less blue up arrow
      556970 Rev. 5
      Antibody Details
      Down Arrow Up Arrow
      1F4

      The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      556970 Rev. 5
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      556970 Rev.5
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "556970" on CiteAb

      Development References (4)

      1. Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Clone-specific: Immunofluorescence). View Reference
      2. Naper C, Vaage JT, Lambracht D, et al. Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control. Eur J Immunol. 1995; 25(5):1249-1256. (Biology). View Reference
      3. Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Clone-specific: Cytotoxicity). View Reference
      4. Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Immunogen: Immunohistochemistry, Immunoprecipitation, Stimulation). View Reference
      View All (4) View Less
      556970 Rev. 5

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.