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Purified Mouse Anti-Human CD89
Purified Mouse Anti-Human CD89
Flow cytometric analysis of CD89 expression on human peripheral blood granulocytes. Human whole blood was stained with either Purified Mouse Anti-Human CD89 (Cat. No. 555685; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of CD89 expression on human peripheral blood granulocytes. Human whole blood was stained with either Purified Mouse Anti-Human CD89 (Cat. No. 555685; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes. Flow cytometry was performed on a BD FACScan™ system.
Product Details
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BD Pharmingen™
FCAR; IgA Fc receptor; FcαR; FcalphaRI; CTB-61M7.2
Human (QC Testing)
Mouse BALB/c IgG1, κ
Purified Human FcαR
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
V MA104
AB_396036
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555685 Rev. 7
Antibody Details
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A59

The A59 monoclonal antibody specifically recognizes CD89. CD89 is the Fc receptor for IgA (FcαR), a 55-75 kDa glycoprotein expressed exclusively on cells of granulocytic and monocyte/macrophage lineages in peripheral blood, but not on lymphocytes. It is also expressed on promyelocytes in bone marrow and in the cytoplasm of fixed monocytes. This mAb does not block IgA binding and is more efficient than native IgA ligands in the isolation of FcαR molecules. FcαR plays a role in triggering phagocytosis and respiratory burst.

555685 Rev. 7
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555685 Rev.7
Citations & References
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View product citations for antibody "555685" on CiteAb

Development References (4)

  1. Monteiro RC, Cooper MD, Kubagawa H. Molecular heterogeneity of Fc alpha receptors detected by receptor-specific monoclonal antibodies. J Immunol. 1992; 148(6):1764-1770. (Biology). View Reference
  2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  3. Shen L, Collins JE, Schoenborn MA, Maliszewski CR. Lipopolysaccharide and cytokine augmentation of human monocyte IgA receptor expression and function. J Immunol. 1994; 152(8):4080-4086. (Biology). View Reference
  4. Shen L. A monoclonal antibody specific for immunoglobulin A receptor triggers polymorphonuclear neutrophil superoxide release. J Leukoc Biol. 1992; 51(4):373-378. (Biology). View Reference
View All (4) View Less
555685 Rev. 7

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.