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BD Phosflow™ Alexa Fluor® 488 Mouse anti-PLCγ1
Clone 10/PLCgamma (RUO)
Analysis of PLCγ1 in human T leukemia cell lines. Jurkat (ATCC TIB-152, open histogram) and J.gamma1 (PLCγ1-deficient Jurkat, ATCC CRL-2678, shaded histogram) were fixed with pre-warmed BD Cytofix™ Fixation buffer (Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 488 Mouse anti-PLCγ1 (Cat. No. 558566). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Analysis of PLCγ1 in human T leukemia cell lines. Jurkat (ATCC TIB-152, open histogram) and J.gamma1 (PLCγ1-deficient Jurkat, ATCC CRL-2678, shaded histogram) were fixed with pre-warmed BD Cytofix™ Fixation buffer (Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 488 Mouse anti-PLCγ1 (Cat. No. 558566). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Analysis of PLCγ1 in human T leukemia cell lines. Jurkat (ATCC TIB-152, open histogram) and J.gamma1 (PLCγ1-deficient Jurkat, ATCC CRL-2678, shaded histogram) were fixed with pre-warmed BD Cytofix™ Fixation buffer (Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 488 Mouse anti-PLCγ1 (Cat. No. 558566). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Analysis of PLCγ1 in human T leukemia cell lines. Jurkat (ATCC TIB-152, open histogram) and J.gamma1 (PLCγ1-deficient Jurkat, ATCC CRL-2678, shaded histogram) were fixed with pre-warmed BD Cytofix™ Fixation buffer (Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 488 Mouse anti-PLCγ1 (Cat. No. 558566). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Product Details
BD Phosflow™
Human (QC Testing), Human, Mouse, Rat, Dog, Chicken (Reactivity Confirmed in Development)
Mouse IgG1
Cow PLCγ1 N-terminal region Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_647093
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
Perm Buffer III RUO
Size
125 ML
Cat No.
558050
Fixation Buffer RUO
Size
100 ML
Cat No.
554655
558566 Rev. 3
Antibody Details
10/PLCgamma
The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCγ1, which is ubiquitously expressed, and 2) PLCγ2, found primarily in the lymphoid system. PLCγ is essential for growth factor-induced cell motility and mitogenesis. PLCγ1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCγ is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCγ activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCγ1, regardless of phosphorylation status. It does not cross-react with PLCγ2.
558566 Rev. 3
Format Details
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
494 nm
517 nm
558566 Rev.3
Citations & References
View product citations for antibody "558566" on CiteAb
Development References (2)
-
Chen P, Murphy-Ullrich JE, Wells A. A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility. J Cell Biol. 1996; 134(3):689-698. (Biology). View Reference
-
Obermeier A, Tinhofer I, Grunicke HH, Ullrich A. Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation. EMBO J. 1996; 15(1):73-82. (Biology). View Reference
558566 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.