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PE Mouse Anti-Human CD54
Product Details
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Human ICAM-1, HICAM-1; BB2; P3.58
Mouse IgG2b, κ
Major histocompatibility Class II antigen negative variant of human B-lymphoblastoid cell line
Flow cytometry
3 μg/mL
20 μL
IV B50, M165
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation And Storage

The monoclonal antibody is supplied as 6 µg purified immunoglobulin in 2.0 mL (3 µg/mL) of phosphate-buffered saline. The PE conjugate is supplied as 6 µg in 2.0 mL (3 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347977 Rev. 1
Antibody Details
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CD54, clone LB-2, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with the major histocompatibility complex (MHC) class II–negative human B-lymphoblastoid cell line 6.16c13.

CD54 (Leu-54) recognizes the intercellular adhesion molecule (ICAM-1), Mr 90 kdaltons (kDa). The CD54 antigen is a ligand for the leucocyte function–associated antigen-1 (CD11a/CD18 [LFA-1]) and influences both LFA-1–dependent adhesion of leucocytes to endothelial cells and immune functions involving cell-to-cell contact.

347977 Rev. 1
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
347977 Rev.1
Citations & References
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View product citations for antibody "347977" on CiteAb

Development References (10)

  1. Bangham CR, McMichael AJ. Nosing ahead in the cold war. Nature. 1990; 344:16. (Biology).
  2. Boyd AW, Dunn SM, Fecondo JV, et al. Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989; 73:1896-1903. (Biology).
  3. Clark EA, Ledbetter JA, Holly RC, Dinndorf PA, Shu G. Polypeptides on human B lymphocytes associated with cell activation. Hum Immunol. 1986; 16(1):100-113. (Biology). View Reference
  4. Dustin ML, Staunton DE, Springer TA. Supergene families meet in the immune system. Immunol Today. 1988; 9:213-215. (Biology).
  5. Johnson JP, Shaw S. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:681-683.
  6. Makgoba MW, Sanders ME, Ginther Luce GE, et al. ICAM-1: a ligand for LFA-1–dependent adhesion of B, T, and myeloid cells. Nature. 1988; 331:86-88. (Biology).
  7. Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber W, Merluzzi VJ. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Nature. 1990; 344:70-72. (Biology).
  8. Patarroyo M, Clark EA, Prieto J, Kantor C, Gahmberg CG. Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB-2. FEBS Lett. 1987 January; 210(2):127-131. (Biology). View Reference
  9. Patarroyo M, Makgoba MW. Leucocyte adhesion to cells. Molecular basis, physiological relevance, and abnormalities.. Scand J Immunol. 1989; 30(2):129-64. (Biology). View Reference
  10. Staunton DE, Dustin ML, Springer TA. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Nature. 1989; 339:61-64. (Biology).
View All (10) View Less
347977 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 


Although not required, these products are manufactured in accordance with Good Manufacturing Practices.