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Anti-Human Lineage Cocktail 2 (lin 2) (CD3, CD14, CD19, CD20, CD56)

Anti-Human Lineage Cocktail 2 (lin 2) (CD3, CD14, CD19, CD20, CD56)

(RUO (GMP))
Product Details
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BD™
40.6 μg/ml
Human
Flow cytometry
RUO (GMP)


Description

The following antibody clones are each composed of mouse IgG1 heavy chains and kappa light chains: CD3, clone SK7 CD19, clone SJ25C1 CD20, clone L27 The following antibody clones are each composed of mouse IgG2b heavy chains and kappa light chains: CD14, clone MφP9 CD56, clone NCAM16.2

The Lineage Cocktail 2 (lin 2) contains antibody clones that, in combination, stain lymphocytes, monocytes, eosinophils, and neutrophils. It includes CD3, CD14, CD19, CD20, and CD56. Peripheral blood dendritic cells (DCs), including those expressing CD16, can be distinguished from other leucocytes by their lack of staining with lin 2.

643397 Rev. 1
Citations & References
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Development References (10)

  1. Banchereau J, Schmitt D, eds. Dendritic Cells in Fundamental and Clinical Immunology, Volume 2. 1995. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Freudenthal PS, Steinman RM. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc Natl Acad Sci USA. 1990; 87:7698-7702. (Biology).
  5. MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). View Reference
  6. Markowicz S, Engleman EG. Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J Clin Invest. 1990; 85:955-961. (Biology).
  7. Olweus J, BitMansour A, Warnke R, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA. 1997; 94:12551-12556. (Biology).
  8. Piccioli D, Tavarini S, Borgogni E, et al. Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. 2007; 109(12):5371-5379. (Biology).
  9. Signorino E, Brusa D, Granata R, Malavasi F, Ferrone S, Matera L. Contribution of Dendritic Cells FcγRI and FcγRIII to Cross-Presentation of Tumor Cells Opsonized with the Anti-MHC Class I Monoclonal Antibodies. Cancer Biol Ther. 6(12)(Biology).
  10. Steinman R M. The dendritic cell system and its role in immunogenicity. Annu Rev Immunol. 1991; 9:271-296. (Biology).
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643397 Rev. 1

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