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      Anti-Human Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)

      BD FastImmune™ Anti-Human Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)

      (RUO (GMP))
      Product Details
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      BD FastImmune™
      140.6 μg/mL
      Human
      Flow cytometry , Intracellular staining (flow cytometry), Northern blot
      RUO (GMP)
      Phosphate buffered saline with gelatin and 0.1% sodium azide.


      Description

      The following are each composed of mouse IgG1 heavy chains and kappa light chains:

      CD3, clone SK7

      CD16, clone 3G8

      CD19, clone SJ25C1

      CD20, clone L27

      The following are each composed of mouse IgG2b heavy chains and kappa light chains:

      CD14, clone MφP9

      CD56, clone NCAM16.2

      The Lineage Cocktail 1 (lin 1) contains antibody clones that, in combination, stain lymphocytes, monocytes, eosinophils, and neutrophils. It includes CD3, CD14, CD16, CD19, CD20, and CD56. Peripheral blood dendritic cells and basophils can be distinguished from other leucocytes by their lack of staining with lin 1.

      Preparation And Storage

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      340546 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD14 FITC MΦP9 IgG2b, κ N/A
      CD56 FITC NCAM16.2 IgG2b, κ N/A
      CD16 FITC 3G8 IgG1, κ N/A
      CD3 FITC SK7 IgG1, κ N/A
      CD20 FITC L27 IgG1, κ N/A
      FITC Mouse anti-Human CD19 SJ25C1 IgG1, κ 930
      340546 Rev. 1
      Citations & References
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      Development References (14)

      1. Banchereau J, Schmitt D, eds. Dendritic Cells in Fundamental and Clinical Immunology, Volume 2. 1995. (Biology).
      2. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
      3. Clinical and Laboratory Standards Institute. 2005. (Biology).
      4. Freudenthal PS, Steinman RM. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc Natl Acad Sci USA. 1990; 87:7698-7702. (Biology).
      5. Grouard G, Durand I, Filgueira L, Banchereau J, Liu YJ. Dendritic cells capable of stimulating T cells in germinal centres.. Nature. 1996; 384(6607):364-7. (Biology). View Reference
      6. Markowicz S, Engleman EG. Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J Clin Invest. 1990; 85:955-961. (Biology).
      7. Mueller R, Heusser CH, Rihs S, Brunner T, Bullock GR, Dahinden CA. Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils. Eur J Immunol. 1994; 24:2935-2940. (Biology).
      8. O'Doherty U, Peng M, Gezelter S, et al. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.. Immunology. 1994; 82(3):487-93. (Biology). View Reference
      9. Olweus J, BitMansour A, Warnke R, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA. 1997; 94:12551-12556. (Biology).
      10. Stain C, Stockinger H, Scharf M, et al. Human blood basophils display a unique phenotype including activation linked membrane structures. Blood. 1987; 70:1872-1879. (Biology).
      11. Steinman R M. The dendritic cell system and its role in immunogenicity. Annu Rev Immunol. 1991; 9:271-296. (Biology).
      12. Thomas R, Lipsky PE. Human peripheral blood dendritic cell subsets. Isolation and characterization of precursor and mature antigen-presenting cells.. J Immunol. 1994; 153(9):4016-28. (Biology). View Reference
      13. Valent P, Besemer J, Muhm M, Majdic O, Lechner K, Bettelheim P. Interleukin 3 activates human blood basophils via high-affinity binding sites. Proc Natl Acad Sci USA. 1989; 86:5542-5546. (Biology).
      14. Valent P. Immunophenotypic characterization of human basophils and mast cells. Chem Immunol. 1995; 61:34-48. (Biology).
      View All (14) View Less
      340546 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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