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PE Phycoerythrin Fluorescence Quantitation Kit

BD Quantibrite™ PE Phycoerythrin Fluorescence Quantitation Kit

Product Details
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Each BD Quantibrite™ PE tube contains a lyophilized pellet of beads conjugated with four levels of phycoerythrin (PE). The pellet is restrained in the bottom of the tube by a stainless steel retainer. The number of PE molecules per bead at each level varies among lots; lot-specific information is included with each kit. Each BD Quantibrite tube contains gelatin and sodium azide and is packaged in a foil pouch. There are 10 tubes per kit.

BD Quantibrite PE tubes are designed for use with PE-labeled monoclonal antibodies for the purpose of estimating ABCs by flow cytometry. When you run a BD Quantibrite PE tube at the same instrument settings as the assay, the FL2 axis can be converted into the number of PE molecules bound per cell. By using known ratios of PE to antibodies, you can then convert PE molecules per cell to antibodies per cell. These factors, and others that affect quantitation, are discussed in detail in the BD Quantibrite white paper.

Preparation And Storage

Store foil pouches at 2°C–8°C and do not freeze. Protect from exposure to light. The reagent is stable until the expiration date shown on the pouch label when stored as directed.

Reconstitute the pellet immediately after removing the tube from the pouch. The reconstituted pellet is stable for 24 hours when protected from light and stored at 2°C– 8°C.

340495 Rev. 1
Citations & References
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Development References (5)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Davis K, Abrams B, Hoffman RA, Bishop JE. Quantitation and valence of antibodies bound to cells. Cytometry. 1996; 150. (Biology).
  4. Iyer S, Bishop J, Abrams, B, et al. QuantiBRITE: A New Standard for Fluorescence Quantitation. Becton Dickinson Immunocytometry Systems. 1997. (Biology).
  5. Iyer S, Suni M, Davis K, Maino V. Expression of CD69 on activated T cells using R-phycoerythrin labeled beads. Cytometry. 1996; #AC78:113. (Biology).
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340495 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 


Although not required, these products are manufactured in accordance with Good Manufacturing Practices.