CD15 FITC

BD™ CD15 FITC

Name: CD15 FITC
Brand: BD™
SKU: 332778

Clone MMA

(CE_IVD)

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Product Details

Brand: BD™

Reactivity: Human

Isotype: Mouse BALB/c IgM, κ

Immunogen: U-937 histiocytic cell line

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 20 μL

Entrez Gene ID: 2526

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: CE_IVD

Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

332778 Rev. 1

Antibody Details

MMA

CD15 is intended for in vitro diagnostic use in the identification of cells expressing CD15 antigen, using a BD FACS™ brand flow cytometer.

The flow cytometer must be equipped to detect light scatter and the appropriate fluorescence, and be equipped with appropriate analysis software (such as BD CellQuest™ or BD LYSYS™ II software) for data acquisition and analysis. Refer to your instrument user’s guide for instructions.

332778 Rev. 1

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

332778 Rev.1

Citations & References

Development References (17)

Andrews RG, Torok-Storb B, Bernstein ID. Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies.. Blood. 1983; 62(1):124-32. (Biology). View Reference
Arber DA, Weiss LM. CD15: a review. Appl Immunohistochem. 1993; 1:17-30. (Biology).
Bernstein ID, Andrews RG, Cohen SF, McMaster BE. Normal and malignant human myelocytic and monocytic cells identified by monoclonal antibodies. J Immunol. 1982; 128(2):876-881. (Biology).
Bettelheim P, Lutz D, Majdic O, al. Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia. Br J Haematol. 1985; 59:395-409. (Biology).
Cantu-Rajnoldi A, Putti C, Saitta M, al. Coexpression of myeloid antigens in childhood acute lymphoblastic leukaemia: relationship with the stage of differentiation and clinical significance. Br J Haematol. 1991; 79:40-43. (Biology).
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. H42-A2. 2007. (Biology).
Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Rothe G, Schmitz G. Leukemia. 1996; 10:877-895. (Biology).
Drexler HG, Sagawa K, Menon M, Minowada J. Reactivity pattern of 'myeloid monoclonal antibodies' with emphasis on MCS-2. Am J Pathol. 1986; 10:17-23. (Biology).
Hanjan SN, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982 May; 23(2):172-188. (Biology). View Reference
Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984; 82:29. (Biology).
Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
NCCLS document. 2001. (Biology).
Pinkus GS, Thomas P, Said JW. Leu-M1—A marker for Reed-Sternberg cells in Hodgkin's disease: an immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985; 119:244. (Biology).
Skubitz K, Balke J, Ball E, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:800-805.
Stelzer GT, Marti G, Hurley A, McCoy PJ, Lovett EJ, Schwartz A. US-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry. 1997; 30:214-230. (Biology).
Van Voorhis WC, Steinman RM, Hair LS, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J Exp Med. 1983; 158:126-145. (Biology).

View All (17)

332778 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

23-22942-00

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.