FlowJo AutoSpill

Fluorescence spillover is the process in which the emission spectra from a fluorochrome are detected not only by its detector but also by other detectors in the flow cytometer. Such overlaps cannot be removed by the optics and must be corrected electronically. Compensation is the process of measuring fluorescence spillover or determining how much of the signal emitted by a fluorochrome attached to a probe is measured by its detector. Improper compensation can seriously affect data quality in flow cytometry, and impact scientific discovery as a consequence.

In this webinar, Dr. John Quinn, Director of Science and Product Development, BD Life Sciences, Bioscience Informatics, introduces the basic principles of traditional compensation using single-stain controls. He then goes on to speak about AutoSpill, a new compensation approach for flow cytometry. While still using single-stain controls, AutoSpill uses robust linear regression to calculate compensation coefficients without identifying negative or positive populations. This approach works even in the absence of good controls. AutoSpill iteratively refines the output to create an optimal matrix and can subtract out auto-fluorescence.

AutoSpill offers a one-button approach to compensation, which is the most tricky part of a flow cytometry experiment. It makes cytometry easier and more convenient.

Watch this webinar on “Autospill: Improved Automation for Cytometric Data” to learn more about AutoSpill and compensation.