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      Purified Mouse Anti-Human CD23
      74691E_550386_image1.png

      Immunohistochemical staining of B lymphocytes. Frozen sections of normal human spleen was reacted with Purified Mouse Anti-Human CD23 (Cat. No. 550386) antibody. B lymphocytes can be identified by the intense brown labeling of their cell surface membranes. Amplification 20X.

      74691E_550386_image1.png Blue-Cap-White-Label.jpg

      Immunohistochemical staining of B lymphocytes. Frozen sections of normal human spleen was reacted with Purified Mouse Anti-Human CD23 (Cat. No. 550386) antibody. B lymphocytes can be identified by the intense brown labeling of their cell surface membranes. Amplification 20X.

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      BD Pharmingen™
      FCER2; FcεRII; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
      62.5 µg/ml
      V CD23.15
      AB_393652
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      Immunohistochemistry: The M-L233 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains B cells and follicular dendritic cells. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the M-L233 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone M-L233 is not recommended for formalin-fixed paraffin embedded sections.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      6. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550386 Rev. 2
      Antibody Details
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      M-L233

      The M-L233 antibody specifically binds to human CD23, the low affinity receptor for human IgE (FcεRII). CD23 is a type II membrane glycoprotein that is expressed by B cells, monocytes, macrophages, eosinophils, platelets and dendritic cells. CD23 mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. Soluble CD23 (sCD23) can be released by CD23-positive cells as a result of proteolytic cleavage of membrane CD23. Larger fragments of sCD23 (e.g., 25-37 kDa) retain their IgE-binding capacity whereas smaller fragments (ie, ≤ 12 kDa) do not. Soluble CD23 may have immunoregulatory effects on the growth and differentiation of B cells and other cell types.

      550386 Rev. 2
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550386 Rev.2
      Citations & References
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      View product citations for antibody "550386" on CiteAb

      Development References (5)

      1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
      2. Delespesse G, Hofstetter H, Sarfati M. Low-affinity receptor for IgE (FcERII, CD23) and its soluble fragments. Int Arch Allergy Immunol. 1989; 90(1):41-44. (Biology). View Reference
      3. Gordon J, Millsum MJ, Flores-Romo L, Gillis S. Regulation of resting and cycling human B lymphocytes via surface IgM and the accessory molecules interleukin-4, CD23 and CD40. Immunology. 1989; 68(4):526-531. (Biology). View Reference
      4. Saeland S, Duvert V, Moreau I, Banchereau J. Human B cell precursors proliferate and express CD23 after CD40 ligation. J Exp Med. 1993; 178(1):113-120. (Biology). View Reference
      5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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      550386 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.