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CITE-seq is a powerful technique that helps unlock deeper biological insights about your samples by combining information from both the transcriptome and proteome.
The complete CITE-seq solution on the BD Rhapsody™ HT Single-Cell Analysis System is now more accessible with the BD® OMICS-One Protein Panels. These SMART-titrated and validated antibody-oligo panels are designed to target key immune cell or tumor markers and can help you uncover immune cell lineages and assess activation and suppression states in your single-cell samples with ease at reduced sequencing costs.
For studies that focus solely on protein expression, BD® OMICS-One Protein Panels also support a protein-only workflow, using the same panels, reagents, instrument and analysis software.
Designed for flexibility, BD® OMICS-One Protein Panels offer a streamlined sample-to-data solution and empower you to generate high-quality protein data, whether focusing solely on protein expression or integrating it with transcriptomic insights
Talk to one of our specialists about bringing BD® OMICS-One Protein Panels to your lab.
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Focused: Lyophilized panels with curated antibody-oligos covering markers for specific areas of immune biology to stain up to 2 million cells
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Flexible: Compatible with other BD® OMICS-One Protein Panels or drop-ins from our growing library of more than 470 single-vial BD® AbSeq Antibody-Oligo Reagents
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SMART: Designed to lower your sequencing cost without compromising sensitivity
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Multiomics enabled: Optimized to work with whole transcriptome, targeted and TCR/BCR profiling RNA-seq assays for multiomic studies
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Intuitive: Supported by the free BD Cellismo™ Data Visualization Tool for data analysis, which requires no bioinformatics expertise or coding experience
Smart Panel Design
SMART Panel Design Lowers Sequencing Cost While Preserving the Sensitivity and Resolution of CITE-Seq Data
CITE-seq or high-plex protein-profiling experiments with antibody-oligos traditionally incur high sequencing costs because highly abundant protein targets such as CD45 and CD44 consume most of the sequencing reads requiring deeper sequencing to detect less abundant secondary and tertiary protein targets. SMART panel design addresses this challenge by utilizing carefully selected, pre-titrated concentrations of antibody-oligos against abundant primary markers. This helps to distribute sequencing reads to the more critical secondary and tertiary targets without compromising the sensitivity or ability to detect the abundant cell surface primary markers.
How does this work? Select antibody-oligos in each BD® OMICS-One Protein Panel that target select, highly expressed primary markers are titrated and lyophilized alongside “cold antibodies” (antibodies lacking oligonucleotides) of the same clone. This approach generates a gradual and predictable decrease in reads allocated to the primary marker targeted thereby moderating the signal of the targeted marker without affecting the ability to resolve cell populations. The outcome is better resolution of low-expressed proteins at the same sequencing depth without incurring additional sequencing costs.
BD® OMICS-One B-Cell Protein Panel
This 30-plex antibody-oligo panel targets key human cell surface protein markers on B-cells, such as CD19, CD20, HLA-DR and CD138. This panel allows you to characterize B-cell subtypes such as plasma cells, IgM memory cells and germinal center B-cells and their activated/suppressed states.
BD® OMICS-One T-Cell Protein Panel
This 30-plex antibody panel targets key human cell surface protein markers on T-cells, such as CD3, CD4, CD8, CD45RO and CDRA. This panel allows you to characterize T-cell subtypes such as naïve, effector and central memory T-cells and their activated/suppressed states.
BD® OMICS-One Tumor Protein Panel
This 30-plex antibody-oligo panel targets key human cell surface protein markers for characterizing tumor cell populations and other features including immune evasion, adhesion and stemness.
BD® OMICS-One NK-Cell Protein Panel
This 30-plex antibody-oligo panel targets key human cell surface protein markers to deeply characterize natural killer (NK) cell phenotypes and functional states and interactions within the innate immune landscape.
BD® OMICS-One APC/Myeloid-Cell Protein Panel
This 30-plex antibody-oligo panel targets key human cell surface protein markers to resolve the heterogeneity, activation states and immunoregulatory functions of antigen-presenting cells (APCs)/myeloid cells.
BD® OMICS-One Immune Profiler Protein Panel
This broad, 30-plex antibody-oligo panel targets major human immune markers allowing you to reliably identify most major immune cell populations in your single cell samples.
BD® OMICS-One Adaptive Protein Panel
This 59-plex antibody-oligo panel targets key human cell surface protein markers for characterizing cell populations and cell states associated with the adaptive immune system.
BD® OMICS-One Innate Protein Panel
This 58-plex antibody-oligo panel targets key human cell surface protein markers to profile the key cell types of the innate immune system within your samples.
BD® OMICS-One Immuno-Oncology Protein Panel
This 85-plex antibody-oligo panel targets key human tumor, T-cell and B-cell surface protein markers. Leverage this panel to study tumor and adaptive immune cell populations in your samples of interest.
BD® OMICS-One Innate Immuno-Oncology Protein Panel
This 83-plex antibody-oligo panel targets key human cell surface protein markers core tumor and innate immune cell markets. Leverage this panel to study the interplay between the innate immune system and tumor biology.
BD® OMICS-One Comprehensive Immuno-Oncology Protein Panel
This 129-plex antibody-oligo panel offers comprehensive marker coverage for major human immune cell types and tumor cells across all BD® OMICS-One Panels for our deepest profiling of sample biology and discovery applications.
The Power of More
A Smart Platform for Your SMART Protein Panels
With our complete solution you can now explore more omes in one data readout. Get more quality data and more breakthroughs, all at less than 1 cent per cell.*
Explore how you can harness the POWER OF MORE with the only complete single-cell multiomics solution.
Request a Consultation
Want to learn more about BD® OMICS-One Protein Panels or our single-cell multiomics solution?
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*1 cent per cell calculation includes instruments and reagents but is not inclusive of sequencing costs.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.