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Overview
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Overview

BD OptiBuild™ On-Demand Reagents make it easy for you to gain access to the antibodies you need on an ever-growing catalog of dye combinations. Whether you want to minimize compensation or add new markers to complex experiments, BD OptiBuild™ Reagents provide flexibility to evaluate new colors and simplify your panel design.

 

Unlike traditional large-scale, expensive custom conjugates, these reagents come in convenient 50-µg vials and can be ordered the same way as any catalog reagent. Products are made especially for you, on-demand and usually ship in less than 72 hours.*

 

*In the continental U.S., Canada, China and Europe.

 
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OptiBuild Overview

Explore how our vast menu of BD OptiBuild™ Reagents can provide flexibility and convenience for your panel design.

Sample Data
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Sample Data

The on-demand nature of BD OptiBuild™ Reagents is the result of years of testing and analysis. We can offer robust reagents that provide a valuable alternative to traditional reagents. The sample data below tell a story of the journey to expand our portfolio of reagents to simplify panel design.

 

Panel design is critically important to generating high-quality data. Principles of panel design dictate the optimal matching of antigen density with fluorochrome brightness, yet at least one required reagent is unavailable as a standard catalog item for approximately 20% of panels designed today. Access to the right reagents can improve resolution and data quality, ultimately leading to deeper scientific insights through your research.  

 

Expanding options for multicolor panels

BD OptiBuild™ Reagents provide choice so that you have multiple options for your markers of interest. BD OptiBuild™ and BD Horizon™ Reagents complement each other so that you can optimize your panel with a reliable reagent in the color you need.

 

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CD21 fluorescence on multiple formats of BD Horizon Brilliant™ and BD OptiBuild™ Reagents

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CD11b fluorescence on multiple formats of BD Horizon Brilliant™ and BD OptiBuild™ Reagents

KLRG1 fluorescence on multiple formats of BD Horizon Brilliant™ and BD OptiBuild™ Reagents

BD Horizon™ BB700: A brighter alternative to PerCP-Cy5.5. The BD OptiBuild™ Reagent portfolio now includes BD Horizon Brilliant™ Blue 700. BB700 was developed as a brighter alternative to PerCP-Cy5.5.

 

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Human CD166 Comparison

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Human CD23 Comparison

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Mouse CD162 Comparison

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Mouse CD38 Comparison

Panel design considerations

Reagent choice brings flexibility to your research, but it’s important to keep in mind the BD Horizon™ Dye principles of matching antigen density with fluorochrome brightness to design optimized panels. Depending on your applications and research goals, some dyes might resolve your populations better than others. The Research Applications team can help refine panel design. For inquiries, email ResearchApplications@bd.com or contact your local reagent support team.

 

CD21 expression on multiple formats of BD Horizon Brilliant™ and BD OptiBuild™ Reagents.

 

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Although BD OptiBuild™ Reagents differ from the way our traditional reagents are manufactured and quality-control tested, these reagents maintain the high performance and quality standards of the BD Horizon Brilliant™ Dyes.

 

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Mouse CD25 direct comparison

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Mouse CD31 direct comparison

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Mouse CD184 direct comparison

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Human CD28 direct comparison

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Human CD14 direct comparison

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Human CD33 direct comparison

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Human CD4 / BD Horizon™ BB700 direct comparison

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Human CD4 / BD Horizon™ BV480 comparison

BD OptiBuild™ Reagent performance in multicolor panels

BD OptiBuild™ Reagents can be used to redesign or expand upon existing panels that have various combinations of fluorochromes and antibodies. BD OptiBuild™ Reagents have been extensively tested in multicolor cocktails to demonstrate reproducible results compared to traditional reagents.

 

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Human 8-color T cell panel direct comparison with and without BD OptiBuild™ Reagents

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Optimized multicolor panel designed with BD OptiBuild™ Reagents

Human 12-color NK cell inhibitory receptor panel designed with BD OptiBuild™ Reagents

 

Violet Blue Red

 

 

Fluorochrome

 

 

Primary Laser Line (nm)

 

 

Marker

 

 

Clone

 

 

Volume/Test

BD Horizon™ BV421 450 CD158f UP-R1 5 µL
BD OptiBuild™ BV480 450 CD85j GHI/75 5 µL
BD OptiBuild™ BV605 450 CD158b CH-L 2.5 µL
BD OptiBuild™ BV711 450 CD94 HP-3D9 0.15 µL
BD OptiBuild™ BV786 450 NKG2a 131411 1.25 µL
BD Pharmingen™ FITC 488 CD57 NK1 20 µL
BD Pharmingen™ PE 488 CD158e1 (NKB1) DX9 20 µL
BD Horizon™ BB700 488 CD27 M-T271 5 µL
BD Pharmingen™ PE-Cy7 488 CD56 B159 5 µL
BD Pharmingen™ APC 640 CD158a HP-3E4 5 µL
BD Pharmingen™ Alexa Fluor™ 700 640 CD16 3G8 5 µL
BD Pharmingen™ APC-H7 640 CD3 SK7 5 µL

Characterization of human NK cells using the NK cell inhibitory receptor panel. 

 

Representative analysis of PBMCs isolated from a healthy human donor. Lymphocytes were first identified based on light scatter properties (not shown). CD3⁻ non-T cells were gated prior to the identification of two major NK subsets: CD56brightCD16⁻ (light green, top histogram) and CD56dimCD16⁺ (dark green, bottom histogram) cells. The expression of differentiation markers and inhibitory receptors was assessed in the two NK cell subsets and revealed expected expression patterns. The sample was acquired on a 3-laser, 12-color BD FACSLyric™ Flow Cytometer. Data were analyzed using FlowJo™ v10 Software.

 

Lot-to-lot consistency

BD OptiBuild™ Reagents are made on demand, which means that every reagent vial is equivalent to a new batch. We know that consistent performance is of the utmost importance for the reliability of research results, so the manufacturing process was also extensively tested for lot-to-lot consistency.

 

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Overlapping histograms of six lots of Ms CD8a BV421 at three concentrations

The data above show multiple conjugation events using the same batches of dye and antibody, demonstrating highly reproducible performance. We also assessed the impact of other potential sources of variability by using different batches of dye and antibody. These reagents will continually perform with minimal lot-to-lot variation so that you can be confident in your results in a multisite or longitudinal study.

 

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Three overlapping histograms of mouse CD8a BV786

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Three overlapping histograms of human CD4 BV421

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Three overlapping histograms of mouse CD28 BV650

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Three overlapping histograms of human CD4 BB700

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Three overlapping histograms of human CD4 BUV395

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Three overlapping histograms of mouse CD8a BV480

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Three overlapping histograms of mouse CD28 BV510

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Three overlapping histograms of human CD19 BV605

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Three overlapping histograms of mouse CD28 BV650

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Three overlapping histograms of human CD4 BV711

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Three overlapping histograms of mouse CD8a BV786

Leveraging the principles of antigen density and relative fluorochrome brightness, BD OptiBuild™ Reagents have been carefully selected to ensure that they can be readily integrated into your ongoing research. The manufacturing process was rigorously validated to ensure that every conjugation produces an active and specific reagent. As with any custom product, there is a minimal possibility that certain permutations do not perform optimally for biological reasons. If you encounter any issues with the performance of these reagents, please contact our Research Applications team.

 

BD OptiBuild™ Reagent data on these clone/format combinations are shown for comparative purposes only, and they are not available for sale. There is no overlap between the BD Horizon™ and BD OptiBuild™ Reagent catalogs.

FAQs
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Frequently Asked Questions

 

Resources
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Related Products

We know that reagent accessibility is important to helping you reach your scientific goals. BD OptiBuild™ Reagents expand your panel design possibilities by using existing antibodies and dyes in combinations not readily available through the standard BD Bioscience reagent portfolio. Our vast menu gives you flexibility in panel design and helps you avoid a timely redesign.

 

Learn more about our fluorochromes and fluorochrome-conjugated antibodies below.

 

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Alexa Fluor is a trademark of Life Technologies Corporation.

Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

CF is a trademark of Biotium, Inc.