BD Rhapsody™ Express Single-Cell Analysis System

A powerful microwell-based single-cell partitioning system

Overview

The BD Rhapsody^™ Express System enables single-cell capture and barcoding of hundreds to thousands of single cells for analysis of genomic and proteomic information, using proprietary, gentle, robust microwell-based single-cell partitioning technology. The BD Rhapsody^™ Express System can be used for lower scale experiments or throughput needs versus the NEW BD Rhapsody^™ HT Xpress System, our NEW platform for flexible and scalable experimental design and higher throughput cell and sample inputs. The BD Rhapsody^™ Express System is also ideal for routine use with familiar cell systems that do not require visual QC or troubleshooting.

Get more information from the BD Rhapsody™ Express System brochure.

Features

The BD Rhapsody™ Express System provides powerful microwell-based single-cell partitioning

Minimal set of benchtop equipment, no fluidic pumps
Allows gentle treatment of cells as they settle into microwells by gravity
Each BD Rhapsody™ Single-Lane Cartridge contains a microwell array with >220,000 partitions
The BD Rhapsody™ Single-Lane Cartridge has a volume ~600 µL; dilute cell suspensions can be loaded directly into the cartridge without needing to concentrate them into very small volumes
No microfluidic channels are involved so clogging of channels with cells is less of a concern
The microwells are specially treated to prevent cells from adhering to surfaces and extensive QC is performed

High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.

Stable magnetic barcoded beads used with the BD Rhapsody™ System can be retained for later experimentation allowing for flexibility with experimental design

BD Rhapsody™ Enhanced Cell Capture Beads remain intact throughout the workflow
Beads with cDNA molecules can be stored for several monthss
Beads can be subsampled for creation of multiple sequencing libraries
Archived beads can be pooled and sequenced together for operational efficiency
Archived beads can be shared easily with research partners
Archived, subsampled beads can help standardize performance and develop SOPs

High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.

High correlation in gene expression seen between technical and biological replicates and between users and sites.

The BD Rhapsody™ Express System is the ideal choice for translational, multi-center, multi-cohort studies

Reliable, reproducible results from gentle, gravity-based single-cell partitioning without microfluidics

Minimal batch effects with technical replicates
Minimal batch effects with biological replicates
Minimal site-to-site variability
Minimal user-to-user variability
Minimum sample biasinimum sample bias
Ability to integrate large data sets without correction for batch effects

High correlation in gene expression seen between technical and biological replicates and between users and sites.

Performance

The BD Rhapsody™ Single Cell Analysis System provides reliable and reproducible results making it an ideal system for translational studies.

The system offers superior cell capture rates and low multiplet rates with a variety of cell types as compared to other systems

Strong correlation of all mRNA and protein markers identifies similar T-cell populations between cartridges

Equivalent data from biological replicates

Equivalent data from subsampled beads

Similar proportions of each cell type are recovered from WTA and targeted analysis on subsampled beads

Applications

The BD Rhapsody™ Single-Cell Analysis System enables simultaneous measurement of surface proteins and mRNA expression, facilitating the identification of distinct subsets of regulatory T cells

Differential gene expression and differential protein expression analysis were performed on three main Treg subsets identified based on manual gating and bivariate analysis of CD45RA and HLA-DR sorted Treg samples. Differential gene expression analysis was performed using the BD Rhapsody™ Targeted Human Immune Response Panel (399 genes). Upregulated genes (>1.25X) are shown in the heat map above. Differential protein expression of 22 proteins was performed simultaneously using BD^® AbSeq Oligo-Conjugated Antibodies.

The BD Rhapsody™ Single-Cell Analysis System can be used for deep characterization of exhausted T cells

Expression of 12 proteins and genes that are significantly upregulated at day 14 of chronic simulation of CD8+ T cells. Two populations (red and green boxes) emerged based on the expression level of markers. Co-expression patterns were discerned for CD38, CD39 and CD94.

The BD Rhapsody™ Single-Cell Analysis System enables resolution of innate lymphoid cell heterogeneity

Differential protein and gene expression at the single-cell level. Heterogeneity was observed in major ILC subsets.

The BD Rhapsody™ Single-Cell Analysis System enables identification of heterogeneity within CLL samples

Clusters are well defined using simultaneous mRNA and protein analysis. Samples from healthy donors cluster similarly. CLL samples cluster separately and uniquely.