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BD Rhapsody™ Express Single-Cell Analysis System
A powerful microwell-based single-cell partitioning system
Overview
The BD Rhapsody™ Express System enables single-cell capture and barcoding of hundreds to thousands of single cells for analysis of genomic and proteomic information, using proprietary, gentle, robust microwell-based single-cell partitioning technology. The BD Rhapsody™ Express System can be used for lower scale experiments or throughput needs versus the NEW BD Rhapsody™ HT Xpress System, our NEW platform for flexible and scalable experimental design and higher throughput cell and sample inputs. The BD Rhapsody™ Express System is also ideal for routine use with familiar cell systems that do not require visual QC or troubleshooting.
Get more information from the BD Rhapsody™ Express System brochure.
Features
The BD Rhapsody™ Express System provides powerful microwell-based single-cell partitioning
- Minimal set of benchtop equipment, no fluidic pumps
- Allows gentle treatment of cells as they settle into microwells by gravity
- Each BD Rhapsody™ Single-Lane Cartridge contains a microwell array with >220,000 partitions
- The BD Rhapsody™ Single-Lane Cartridge has a volume ~600 µL; dilute cell suspensions can be loaded directly into the cartridge without needing to concentrate them into very small volumes
- No microfluidic channels are involved so clogging of channels with cells is less of a concern
- The microwells are specially treated to prevent cells from adhering to surfaces and extensive QC is performed
Stable magnetic barcoded beads used with the BD Rhapsody™ System can be retained for later experimentation allowing for flexibility with experimental design
- BD Rhapsody™ Enhanced Cell Capture Beads remain intact throughout the workflow
- Beads with cDNA molecules can be stored for several monthss
- Beads can be subsampled for creation of multiple sequencing libraries
- Archived beads can be pooled and sequenced together for operational efficiency
- Archived beads can be shared easily with research partners
- Archived, subsampled beads can help standardize performance and develop SOPs
High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.
The BD Rhapsody™ Express System is the ideal choice for translational, multi-center, multi-cohort studies
Reliable, reproducible results from gentle, gravity-based single-cell partitioning without microfluidics
- Minimal batch effects with technical replicates
- Minimal batch effects with biological replicates
- Minimal site-to-site variability
- Minimal user-to-user variability
- Minimum sample biasinimum sample bias
- Ability to integrate large data sets without correction for batch effects
High correlation in gene expression seen between technical and biological replicates and between users and sites.
Performance
The BD Rhapsody™ Single Cell Analysis System provides reliable and reproducible results making it an ideal system for translational studies.
The system offers superior cell capture rates and low multiplet rates with a variety of cell types as compared to other systems
Strong correlation of all mRNA and protein markers identifies similar T-cell populations between cartridges
Equivalent data from biological replicates
Equivalent data from subsampled beads
Similar proportions of each cell type are recovered from WTA and targeted analysis on subsampled beads
The BD Rhapsody™ Single-Cell Analysis System enables simultaneous measurement of surface proteins and mRNA expression, facilitating the identification of distinct subsets of regulatory T cells
Differential gene expression and differential protein expression analysis were performed on three main Treg subsets identified based on manual gating and bivariate analysis of CD45RA and HLA-DR sorted Treg samples. Differential gene expression analysis was performed using the BD Rhapsody™ Targeted Human Immune Response Panel (399 genes). Upregulated genes (>1.25X) are shown in the heat map above. Differential protein expression of 22 proteins was performed simultaneously using BD® AbSeq Oligo-Conjugated Antibodies.
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The BD Rhapsody™ Single-Cell Analysis System can be used for deep characterization of exhausted T cells
Expression of 12 proteins and genes that are significantly upregulated at day 14 of chronic simulation of CD8+ T cells. Two populations (red and green boxes) emerged based on the expression level of markers. Co-expression patterns were discerned for CD38, CD39 and CD94.
The BD Rhapsody™ Single-Cell Analysis System enables resolution of innate lymphoid cell heterogeneity
Differential protein and gene expression at the single-cell level. Heterogeneity was observed in major ILC subsets.
The BD Rhapsody™ Single-Cell Analysis System enables identification of heterogeneity within CLL samples
Clusters are well defined using simultaneous mRNA and protein analysis. Samples from healthy donors cluster similarly. CLL samples cluster separately and uniquely.
BD Rhapsody™ Express Single-Cell Analysis System
A powerful, high-throughput, microwell-based single-cell partitioning system
- Brochures
- User Guides
- Data Sheets
- Integration of the Element AVITI System with BD Rhapsody Single-Cell Assays
- A Comprehensive Characterization of Regulatory T Cells Using the BD Rhapsody™ Single-Cell Analysis System
- Assessing Obesity-Induced Inflammation in Mice through Single-Cell Multiomic Analysis
- Exploring Tumor Heterogeneity of Chronic Lymphocytic Leukemia Using Single-Cell Multiomics
- Resolving Human Circulating Innate Lymphoid Heterogeneity Using Advanced Single-Cell Multiomic Analysis
- Streamlined Transfer of High-Parameter Flow Cytometry Panel from Cell Analyzer to Cell Sorter Prior to In-Depth Single-Cell Multiomic Analysis of Murine Lymphocyte Subpopulations
- Leveraging the Power of High-Parameter Cell Sorting and Single-Cell Multiomics to Profile Intratumoral Immune Cells in a Model of B-Cell Lymphoma
- Presentations
- CLL Phenotyping with BD Rhapsody™ Targeted Panels and BD® AbSeq Assays
- Single-Cell Multiomic Analysis for Deep Characterization of Exhausted T Cells
- Single-Cell Multiomic Analysis of Immune Cells in Obese Mice
- One Solution for Comprehensive Treg Characterization Via Single-Cell Multiomic Analysis
- Single-Cell Multiomic Analysis for the Resolution of Innate Lymphoid Cell Heterogeneity
- Protocols
- Technical Note
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Results shown are not guaranteed and may not be representative.
Dynabeads is a trademark of Thermo Fisher Scientific.