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Introducing BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents: The superior alternative to PE and PerCP tandem dyes
Our next-generation fluorochromes offer:
- Reduced background: significant reduction in monocyte background compared to PE-Cy tandems
- Range of brightness: a range of fluorochromes to support the detection of surface and intracellular markers from a variety of expression levels
- Versatility: compatible with a variety of common fixation and permeabilization systems, viability dyes and anti-coagulants
- Reliable performance: stable reagents that are photostable and offer lot-to-lot consistency
- Panel design flexibility: use RealYellow and RealBlue Reagent Pairs together on appropriately equipped instruments to build larger panels and for added flexibility when designing panels on both conventional and spectral flow cytometers.
Use the next-generation laser-specific flow cytometry research reagents driven by the new BD dye technology specifically engineered to overcome the challenges of PE tandems. See how:
| Format | Spectral | Conventional | Relative Brightness | Spillover (1-low, 4-high) | Alternative To |
|---|---|---|---|---|---|
| Blue Laser Line | |||||
| RB545 | ✓ | 1 | Unique position | ||
| RB613 | ✓ | ✓ | 2 | PE-CF594*, PE-Dazzle™ 594* or BB630-P2 | |
| RB705 | ✓ | ✓ | 2 | PerCP-Cy5.5 or BB700 | |
| RB744 | ✓ | ✓ | 1 | BB755-P | |
| RB780 | ✓ | ✓ | 1 | PE-Cy7* or BB7990-P | |
| Yellow-Green Laser Line | |||||
| RY586 | ✓ | ✓ | 1 | PE** | |
| RY610 | ✓ | ✓ | 1 | PE-CF594** or PE-Dazzle™ 594** | |
| RY703 | ✓ | ✓ | 2 | PE-Cy5.5** or StarBright™ Yellow 720 | |
| RY775 | ✓ | ✓ | 2 | PE-Cy7** or StarBright™ Yellow 800 | |
* On three-laser configuration (B, V, R)
** On four- or five-laser configuration (B, V, R, YG or UV, V, B, YG, R)
Compared to PE-Cy tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer reduced background.
BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7
RY775 provides improved nonspecific binding.
Compared to PE and PerCP tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer significantly reduced spillover spread.
RY610 shows reduced spillover spread into the corresponding blue detectors.
RB613 shows reduced spillover spread into the corresponding yellow-green detectors.
BD Horizon RealYellow™ 586 Reagents offer high resolution with minimal cross-laser excitation. Data was acquired on a BD FACSymphony™ A5 SE Cell Analyzer.
Unlike select PE tandems such as PE-Cy7, PE-Cy5.5, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer the detection of low-expression surface and intracellular markers.
For more details on each of the dyes, visit the BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents page.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.