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Overview

Next-generation laser-specific flow cytometry research reagents


The family of BD Horizon RealYellow and BD Horizon RealBlue Reagents is bright, clean and laser specific to help streamline the path to your scientific discoveries.

 

Driven by next-generation BD dye technology and AI-guided fluorochrome selection to optimize spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover to optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

RB545
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BD Horizon RealBlue™ 545 (RB545) Reagents

RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:

 

  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers

 

Download the BD Horizon RealBlue™ 545 Brochure>

 
RB545 vials
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RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

Performance

BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

RB545 Normalized Emission Filter Parameters
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RB545 Spread Plots
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Applications

BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
CellBlox™ Blocking BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
 
RB613
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BD Horizon RealBlue™ 613 (RB613) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

 

RB613 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

 

  • A bright fluorochrome to support the detection of low-expression surface and intracellular markers
  • Significantly reduced cross-laser excitation off the 561-nm yellow-green laser
  • Compatibility with conventional and spectral flow cytometers with the appropriate detectors
 
RB613 vials
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RB613 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

 

RB613 reagents increase panel design flexibility off the 488-nm blue laser, offering significantly reduced cross-laser excitation in hundreds of antibody specificities.

Performance

BD Horizon RealBlue™ 613 Reagents are the best choice in this position for flow cytometers with a 488-nm blue laser

RB613 and PE-CF594 Normalized Emission Profile
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Normalized emission profile of RB613 and PE-CF594 fluorochromes.

RB613 Reduced Spillover Spread
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RB613 shows reduced spillover spread into the corresponding yellow-green detectors.

Applications

BD Horizon RealBlue RB613 Regents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
 
RB705
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BD Horizon RealBlue™ 705 (RB705) Reagents

 

The superior alternative to PerCP-Cy5.5 and BD Horizon Brilliant™ Blue 700 (BB700) Reagents

 

RB705 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

 

  • A bright fluorochrome to support the detection of low-expression surface and intracellular markers 
  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Reduced background compared to BB700 with no additional wash recommended

 

Download the BD Horizon RealBlue705 Brochure>

 
RB705 Product shot
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RB705 reagents can be used instead of PerCP-Cy5.5 or BB700 on conventional flow cytometers or with PerCP-Cy5.5 or BB700 on spectral flow cytometers

 

RB705 reagents increase panel design flexibility off the 488-nm blue laser, offering minimal cross-laser excitation in hundreds of antibody specificities.

Performance

BD Horizon RealBlue™ 705 Reagents provide minimal cross-laser excitation off the 561-nm yellow-green laser

 

RB705 Normalized Emission Profile
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Normalized emission profile of RB705, BB700 and PerCP-Cy5.5 fluorochromes.

Spillover comparison of PerCP-Cy5.5 and BB700 with RB705
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Compared to PerCP-Cy5.5 and BB700, RB705 shows lower spillover spread into the corresponding yellow-green, red, UV and violet detectors.

Applications

BD Horizon RealBlue™ 705 Regents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
CellBlox™ Blocking BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
 
RB744
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BD Horizon RealBlue™ 744 (RB744) Reagents

 

A bright new option for high-parameter and spectral flow cytometry panels

 

RB744 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

 

  • A bright fluorochrome to support the detection of low-expression surface and intracellular markers 
  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Compatibility with appropriately configured* BD FACSymphony™ Analyzers and spectral flow cytometers
 
*Filter centered near 750 nm, e.g., 750/30
 
Download the BD Horizon RealBlue™ 744 Reagent brochure >
 
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RB744 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

 

RB744 reagents increase panel design flexibility off the 488-nm blue laser, offering minimal cross-laser excitation in hundreds of antibody specificities.

Performance

BD Horizon RealBlue™ 744 Reagents provide minimal cross-laser excitation off the 561-nm yellow-green laser

RB744 Normalized Emission
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Normalized emission profile of RB744 fluorochromes

RB744 Minimal Spillover Spread
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RB744 shows minimal spillover spread into the corresponding yellow-green, red, UV and violet detectors.

Applications

BD Horizon RealBlue™ 744 Regents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
CellBlox™ Blocking BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
 
RB780
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BD Horizon RealBlue™ 780 (RB780) Reagents—the superior alternative to PE-Cy7

RB780 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488 nm blue laser to offer:

 

  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Bright fluorochrome to support the detection of low-expression surface and intracellular markers 
  • Reduced monocyte background compared to PE-Cy7

 

Download the BD Horizon RealBlue™ 780 Reagents Brochure >

 
RB-780 video
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RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers

 

RB780 reagents can be used with PE-Cy7 on appropriately configured conventional and spectral cytometers with both a 488-nm blue and 561-nm yellow-green laser to increase the number of experimental parameters.

Performance

BD Horizon™ RB780 Reagents offer minimal cross-laser excitation off the yellow-green laser and reduced background compared to PE-Cy7

RB-780 Emission Profile
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Normalized emission profile of PE-Cy7 and RB780 with RB780 minimal excitation off the 561-nm yellow-green laser.

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Human whole blood was stained with BD Horizon™  RB780 Human CD4 (SK3) Reagent, acquired and compensated on a BD FACSymphony  A5 SE Cell Analyzer.

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BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

Applications

BD Horizon RealBlue™ 780 Reagents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
CellBlox™ Blocking BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
 
RY586
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Introducing
BD Horizon RealYellow™ 586 (RY586) Reagents

BD Horizon RealYellow™ 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. They are excited primarily by the 561-nm yellow-green laser and offer:

 

  • Minimal cross-laser excitation off the 488-nm blue laser
  • Bright fluorescence to detect low-expression surface and intracellular markers 

 

Conventional flow cytometry: Just as bright as PE, but with significantly less cross-laser excitation, RY586 reagents can be used instead of PE on conventional flow cytometers for more flexible panel design.

 

Spectral flow cytometry: With a distinct spectral profile from PE, RY586 reagents can be used with PE on spectral flow cytometers to increase parameters for deep scientific insights.

 
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Excellent Resolution

Generate reliable data that clearly resolve even the most challenging cell populations by leveraging bright, clean fluorochromes that minimize spillover and background.

Flexible Panel Design

Maximize panel design options for both conventional and spectral flow cytometry by using reagents that deliver minimal cross-laser excitation in hundreds of antibody specificities.

High-Dimensional Analysis

Reveal more insights from the same sample by expanding the utility of blue and yellow-green laser channels with reagents that enable the simultaneous use of more detectors.

Performance

Click through the slides below to view performance data.

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“The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

 

“The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

 

Dr. Florian Ebner
Distinguished Lab Scientist
Cancer Pharmacology and Disease Positioning

“I’m really excited about the BD Horizon RealBlue 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

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“I’m really excited about the BD Horizon RealBlue 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

Johannes Brandi
Researcher
Bernhard Nocht Institute for Tropical Medicine

"BD Horizon RealYellow™ 586 (RY586) Reagents look like the best alternative for PE and will simplify panel design on conventional flow cytometers. They can also be an additional dye for spectral panel design.”

"BD Horizon RealYellow™ 586 (RY586) Reagents look like the best alternative for PE and will simplify panel design on conventional flow cytometers. They can also be an additional dye for spectral panel design.”

Gert Van Isterdael, PhD
VIB Ghent University, Belgium
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BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents


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BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. CellBlox is a trademark of Thermo Fisher Scientific.