BD Horizon™ Real Fluorochromes

Minimize Cross-laser Spillover. Optimize Panel Resolution.

Format	Spectral	Conventional	Spillover (1-low, 4-high)	Alternative To
Violet Laser
RV828	✓	‒	1	Qdot^™ 800, NovaFluor^™ V800
Blue Laser
RB545	✓	‒	1	(Unique position)
RB613	✓	✓	2	PE-CF594, PE-Dazzle^™ 594, BB630-P2, StarBright^™ Blue 615
RB670	✓	✓	2	PE-Cy5, BB660-P2, StarBright^™ Blue 675
RB705	✓	✓	2	PerCP-Cy5.5, BB700, StarBright^™ Blue 700
RB744	✓	✓	1	BB755-P
RB780	✓	✓	1	PE-Cy7, BB790-P
RB824	✓	✓	1	PerCP/Fire^™806, StarBright^™Blue 810
Yellow-Green Laser
RY586	✓	✓	1	PE
RY610	✓	✓	1	PE-CF594, PE-Dazzle^™ 594
RY655	✓	✓	3	PE-Cy5, StarBright Yellow 665
RY703	✓	✓	2	PE-Cy5.5, StarBright^™ Yellow 720
RY743	✓	✓	2	PE/Fire^™ 744
RY775	✓	✓	2	PE-Cy7, StarBright^™ Yellow 800
Red Laser
RR688	✓	‒	1	Alexa Fluor^™ 660, Spark NIR^™685, NovaFluor^™ R685, cFluor^™ R685

“The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

Dr. Florian Ebner

Distinguished Lab Scientist

Cancer Pharmacology and Disease Positioning

“I’m really excited about the BD Horizon RealBlue^™ 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

Johannes Brandi

Researcher

Bernhard Nocht Institute for Tropical Medicine

"BD Horizon RealYellow™ 586 (RY586) Reagents look like the best alternative for PE and will simplify panel design on conventional flow cytometers. They can also be an additional dye for spectral panel design.”