BD Transduction Laboratories™ Purified Mouse Anti-Brm
Clone 24/BRM (RUO)
Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Brm antibody.
Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Brm antibody.
Immunofluorescence staining of a rabbit lung section.
Immunofluorescence staining of a rabbit lung section.
Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Brm antibody.
Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Brm antibody.
Immunofluorescence staining of a rabbit lung section.
Immunofluorescence staining of a rabbit lung section.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. One of these proteins, SNF2/SWI2, has both Drosophila and mammalian homologues, designated brm. The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. The cooperation between Brm and GR requires the DNA binding domain of GR and the helicase domain and the P/Q-charged domain of Brm. However, Brm does not affect on several other transcription factors. The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. Like Brm, Rb up-regulates glucocorticoid-receptor-mediated transcription. Brm and Rb interact in vitro and in vivo, requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Muchardt C, Yaniv M. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor. EMBO J. 1993; 12(11):4279-4290. (Biology). View Reference
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Singh P, Coe J, Hong W. A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor. Nature. 1995; 374(6522):562-565. (Biology). View Reference
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Wong AK, Shanahan F, Chen Y, et al. BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines. Cancer Res. 2000; 60(21):6171-6177. (Biology: Western blot). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.